Anti-SCD1抗体[CD.E10] (ab19862)

概述

  • 产品名称
    Anti-SCD1抗体[CD.E10]
    参阅全部 SCD1 一抗
  • 描述
    小鼠单克隆抗体[CD.E10] to SCD1
  • 经测试应用
    适用于: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WB, IP, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein (Human).

  • 阳性对照
    • NIH 3T3 cells
  • 常规说明
    SCD1 is known to undergo post-translational modifications and the sizes differ in different cell lines so the observed band size can be different than predicted band size. As positive control we recommend using SCD1 over-expressed 293 transfected cell lysates for western blot.

性能

应用

Our Abpromise guarantee covers the use of ab19862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 4 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 20530718
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB 1/1000. Predicted molecular weight: 41.5 kDa. PubMed: 18697866
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

靶标

  • 功能
    Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA.
  • 序列相似性
    Belongs to the fatty acid desaturase family.
  • 结构域
    The histidine box domains may contain the active site and/or be involved in metal ion binding.
  • 细胞定位
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACOD_HUMAN antibody
    • Acyl-CoA desaturase antibody
    • Delta(9)-desaturase antibody
    • Delta-9 desaturase antibody
    • Delta-9-Desaturase antibody
    • FADS5 antibody
    • Fatty acid desaturase antibody
    • PRO0998 antibody
    • SCD 1 antibody
    • SCD antibody
    • SCD1 antibody
    • Stearoyl-CoA desaturase antibody
    see all

图片



  • Predicted band size : 41.5 kDa


    Western blot using ab19862 on 293 cells.
  • SCD was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Mouse monoclonal to SCD and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab19862.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 32kDa; SCD.
  • ab19862 staining SCD in human skin.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ICC/IF image of ab19862 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19862, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HepG2 cells stained with ab19862 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19862, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

文献

This product has been referenced in:
  • Wu G  et al. Menin enhances c-Myc-mediated transcription to promote cancer progression. Nat Commun 8:15278 (2017). WB ; Human . Read more (PubMed: 28474697) »
  • Paiardi C  et al. The Stearoyl-CoA Desaturase-1 (Desat1) in Drosophila cooperated with Myc to Induce Autophagy and Growth, a Potential New Link to Tumor Survival. Genes (Basel) 8:N/A (2017). Read more (PubMed: 28452935) »

See all 27 Publications for this product

客户评价及客户问答

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Application
Flow Cytometry
Fixation
Formaldehyde
Permeabilization
Yes - 0.1% PBS-Tween
Sample
Rat Cell (liver)
Specification
liver
Gating Strategy
CD24 CELLS
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 0.1% PBS BSA
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Abcam user community

Verified customer

提交于 Dec 10 2013

Application
Flow Cytometry
Fixation
Formaldehyde
Permeabilization
Yes - BD PERMEABILISATION BUFFER
Sample
Human Cell (HEP G2)
Specification
HEP G2
Gating Strategy
CD24 CELLS
Preparation
Cell harvesting/tissue preparation method: single cell suspension
Sample buffer: 0.1% PBS BSA
Username

Abcam user community

Verified customer

提交于 Dec 06 2013

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (8)
Sample
Human Cell lysate - whole cell (LIVER)
Specification
LIVER
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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Verified customer

提交于 Dec 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Rat kidney tissue)
Loading amount
140 µg
Specification
Rat kidney tissue
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

提交于 Aug 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2)
Loading amount
30 µg
Specification
HepG2
Gel Running Conditions
Reduced Denaturing (4-12%Nupage)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Mar 01 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin sections)
Specification
Skin sections
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3%
Username

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Verified customer

提交于 Jan 15 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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