概述

  • 产品名称Anti-SCD1抗体
    参阅全部 SCD1 一抗
  • 描述
    兔多克隆抗体to SCD1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human, Pig
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SCD.

    (Peptide available as ab40137.)

  • 阳性对照
    • This antibody gave a positive signal in HEK293 (Human embryonic kidney cell line) and HepG2 (Human hepatocellular liver carcinoma cell line) whole cell lysates.

性能

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应用

Our Abpromise guarantee covers the use of ab39969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 41.5 kDa).

靶标

  • 功能Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA.
  • 序列相似性Belongs to the fatty acid desaturase family.
  • 结构域The histidine box domains may contain the active site and/or be involved in metal ion binding.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACOD_HUMAN antibody
    • Acyl-CoA desaturase antibody
    • Delta(9)-desaturase antibody
    • Delta-9 desaturase antibody
    • Delta-9-Desaturase antibody
    • FADS5 antibody
    • Fatty acid desaturase antibody
    • PRO0998 antibody
    • SCD 1 antibody
    • SCD antibody
    • SCD1 antibody
    • Stearoyl-CoA desaturase antibody
    see all

Anti-SCD1 antibody 图像

  • All lanes : Anti-SCD1 antibody (ab39969) at 2 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 41.5 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab39969 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39969, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SCD1 antibody (ab39969)参考文献

ab39969 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Pig Tissue lysate - other (Adipose tissue)
Loading amount 8 µg
Specification Adipose tissue
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Dec 18 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"