ab64828 detects endogenous levels of total SCAND1 protein.
Synthetic peptide derived from an internal sequence of human SCAND1.
- Jurkat cell extracts and HepG2 cells.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
ab64828 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Predicted molecular weight: 19 kDa.
||1/500 - 1/1000.
May regulate transcriptional activity.
Contains 1 SCAN box domain.
Information by UniProt
- Leap1 antibody
- Leucine rich domain interacting protein 1 antibody
- Pgc2 antibody
Immunocytochemistry/ Immunofluorescence - SCAND1 antibody (ab64828)
ab64828, at 1/500 dilution, staining SCAND1 in HepG2 cells by Immunofluorescence, in the absence (left image) or presence (right image) of the immunising peptide.
Western blot - SCAND1 antibody (ab64828)
All lanes :
Anti-SCAND1 antibody (ab64828) at 1/500 dilutionLane 1 :
Jurkat cell extract at 5 µgLane 2 :
Jurkat cell extract plus immunising peptide at 5 µgPredicted band size:
19 kDaObserved band size:
25 kDa (why is the actual band size different from the predicted?
)Additional bands at:
70 kDa (possible isoform)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"