Anti-SAF-B2抗体(ab104220)
Key features and details
- Rabbit polyclonal to SAF-B2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SAF-B2抗体
参阅全部 SAF-B2 一抗 -
描述
兔多克隆抗体to SAF-B2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human SAF-B2 aa 351-502.
Database link: AAH25279 -
阳性对照
- 293T, A431 and Jurkat cell lines. HBL435 xenograft.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab104220于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 107 kDa.
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IHC-P |
1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer ( PH8.0).
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 107 kDa. |
IHC-P
1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer ( PH8.0). |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Binds to scaffold/matrix attachment region (S/MAR) DNA. Can function as an estrogen receptor corepressor and can also inhibit cell proliferation. -
组织特异性
Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines. -
序列相似性
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SAP domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 9667 Human
- Omim: 608066 Human
- SwissProt: Q14151 Human
- Unigene: 655392 Human
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别名
- DKFZP779C1727 antibody
- HAP antibody
- HET antibody
see all
图片
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Anti-SAF-B2 antibody (ab104220) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 107 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed A431, using ab104220 at 1/200 dilution. Lower picture costained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using ab104220 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab104220 尚未被引用在任何文献中。