Anti-Sca1 / Ly6A/E抗体[EPR3355] (ab109211)


  • 产品名称Anti-Sca1 / Ly6A/E抗体[EPR3355]
    参阅全部 Sca1 / Ly6A/E 一抗
  • 描述
    兔单克隆抗体[EPR3355] to Sca1 / Ly6A/E
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide corresponding to residues in Mouse Sca1/Ly6A/E.

  • 阳性对照
    • Mouse kidney cell lysate; Mouse kidney tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab109211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能T-cell activation.
    • 组织特异性Widely expressed.
    • 序列相似性Contains 1 UPAR/Ly6 domain.
    • 翻译后修饰O-glycosylated. Not N-glycosylated.
      Not phosphorylated.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Ly-6A.2/Ly-6E. antibody
      • Ly-6A.2/Ly-6E.1 antibody
      • Ly6a antibody
      • LY6A_MOUSE antibody
      • Ly6al antibody
      • Lymphocyte antigen 6A-2/6E-1 antibody
      • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
      • SCA-1 antibody
      • Stem cell antigen 1 antibody
      • T cell activating protein antibody
      • T-cell-activating protein antibody
      • TAP antibody
      see all

    Anti-Sca1 / Ly6A/E antibody [EPR3355] 图像

    • Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211) at 1/1000 dilution + Mouse kidney cell lysate at 10 µg

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 14 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    • ab109211, at 1/50 dilution staining Sca1 / Ly6A/E in paraffin-embedded Mouse kidney tissue by Immunohistochemistry.
    • Immunohistochemical analysis of murine bone marrow tissue, following total body irradiation, staining Sca1 / Ly6A/E with ab109211. Sections were blocked with blocking reagent before staining with primary antibody and a peroxidase conjugated anti-rabbit IgG. Staining was detected with AEC substrate.

    Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)参考文献

    This product has been referenced in:
    • Okamoto M  et al. Stem Cell Antigen 1-Positive Mesenchymal Cells Are the Origin of Follicular Cells during Thyroid Regeneration. PLoS One 8:e80801 (2013). IHC-P ; Mouse . Read more (PubMed: 24278321) »
    • Basile LA  et al. HemaMax™, a recombinant human interleukin-12, is a potent mitigator of acute radiation injury in mice and non-human primates. PLoS One 7:e30434 (2012). IHC-P ; Mouse . Read more (PubMed: 22383962) »

    See all 2 Publications for this product

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    Thank you for contacting us. I have heard back from the lab: For WB, denaturing and reducing conditions were used. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.