Anti-Ly-6A/E (Sca-1)抗体[E13 161-7] - Hematopoietic Stem Cell Marker (ab51317)
Key features and details
- Rat monoclonal [E13 161-7] to Ly-6A/E (Sca-1) - Hematopoietic Stem Cell Marker
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse
- Isotype: IgG2a
概述
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产品名称
Anti-Ly-6A/E (Sca-1)抗体[E13 161-7] - Hematopoietic Stem Cell Marker
参阅全部 Ly-6A/E (Sca-1) 一抗 -
描述
大鼠单克隆抗体[E13 161-7] to Ly-6A/E (Sca-1) - Hematopoietic Stem Cell Marker -
宿主
Rat -
经测试应用
适用于: Flow Cyt, ICC/IFmore details
不适用于: WB -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: Mouse TH2 cell line. Flow Cyt: C57 BL/6 mouse splenocytes
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team. -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
E13 161-7 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51317于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 0.2µg for 106 cells.
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ICC/IF | (2) |
Use a concentration of 5 µg/ml.
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说明 |
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Flow Cyt
Use 0.2µg for 106 cells. |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
T-cell activation. -
组织特异性
Widely expressed. -
序列相似性
Contains 1 UPAR/Ly6 domain. -
翻译后修饰
O-glycosylated. Not N-glycosylated.
Not phosphorylated. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 110454 Mouse
- SwissProt: P05533 Mouse
- Unigene: 425949 Mouse
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别名
- Ly-6A.2/Ly-6E. antibody
- Ly-6A.2/Ly-6E.1 antibody
- Ly6a antibody
see all
图片
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ab51317 stained mouse TH2 cells. The cells were 4% formaldehyde fixed for 4 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51317 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165) used at a 1/1000 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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Flow cytometry staining of C57 BL/6 mouse splenocytes with ab51317 (right) or Rat IgG2aκ (ab18450) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % mouse serum/10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab51317) or Rat IgG2aκ (ab18450) isotype (1x 106 in 100μl at 0.2μg/ml) for 30 min on ice.
The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD4.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on live lymphocytes.
数据表及文件
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SDS download
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Datasheet download
文献 (32)
ab51317 被引用在 32 文献中.
- Sun W et al. SENP1 regulates the transformation of lung resident mesenchymal stem cells and is associated with idiopathic pulmonary fibrosis progression. Cell Commun Signal 20:104 (2022). PubMed: 35836260
- Zhou X et al. SM22α-lineage niche cells regulate intramembranous bone regeneration via PDGFRβ-triggered hydrogen sulfide production. Cell Rep 39:110750 (2022). PubMed: 35508129
- Figeac F et al. Impaired Bone Fracture Healing in Type 2 Diabetes Is Caused by Defective Functions of Skeletal Progenitor Cells. Stem Cells 40:149-164 (2022). PubMed: 35257177
- Ford MJ et al. Oviduct epithelial cells constitute two developmentally distinct lineages that are spatially separated along the distal-proximal axis. Cell Rep 36:109677 (2021). PubMed: 34496237
- Guo S et al. GATA4-driven miR-206-3p signatures control orofacial bone development by regulating osteogenic and osteoclastic activity. Theranostics 11:8379-8395 (2021). PubMed: 34373748