Anti-SC35抗体[SC-35] -核Speckle Marker (ab11826)


  • 产品名称Anti-SC35抗体[SC-35] -核Speckle Marker
    参阅全部 SC35 一抗
  • 描述
    小鼠单克隆抗体[SC-35] to SC35 -核Speckle Marker
  • 特异性In immunohistology and immunoelectronmicroscopy, the antibody labels SC-35 as a speckled pattern that occupies a portion of the nucleoplasm, excluding the nucleoli. Nie et al (2002) report that "In human endometrium the SC35 protein was detected by western blot analysis and had a molecular mass of approximately 90 kDa, indicating that in the endometrial extracts, the 35 kDa protein remains bound to one or more components of the spliceosome."
  • 经测试应用适用于: ICC, ICC/IF, Other, IHC-P, ELISA, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Mouse, Rat, Human, Xenopus laevis, Drosophila melanogaster, Rhesus monkey, Newt
  • 表位This antibody recognizes a phospho-epitope of the non-snRNP (small nuclear ribonucleoprotein particles) factor SC35. The antibody reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF.
  • 阳性对照
    • Cultured human fibroblasts. This antibody gave a positive signal in the following cell types: HeLa. (IF)



Our Abpromise guarantee covers the use of ab11826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/2000.
ICC/IF Use a concentration of 1 µg/ml.
Other Use at an assay dependent concentration.
IHC-P 1/500.
ELISA Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
IP Use at an assay dependent concentration. See Fu and Maniatis 1992 reference.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway.
    • 序列相似性Belongs to the splicing factor SR family.
      Contains 1 RRM (RNA recognition motif) domain.
    • 翻译后修饰Extensively phosphorylated on serine residues in the RS domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 35 kDa antibody
      • arginine/serine-rich 2 antibody
      • PR264 antibody
      • Protein PR264 antibody
      • SC 35 antibody
      • SC-35 antibody
      • SC35 antibody
      • Serine/arginine-rich splicing factor 2 antibody
      • SFRS 2 antibody
      • SFRS2 antibody
      • SFRS2A antibody
      • Splicing component 35 kDa antibody
      • Splicing component antibody
      • Splicing factor antibody
      • Splicing factor arginine/serine rich 2 antibody
      • Splicing factor SC35 antibody
      • Splicing speckle antibody
      • Splicing speckles antibody
      • SR splicing factor 2 antibody
      • SRp30b antibody
      • SRSF2 antibody
      • SRSF2_HUMAN antibody
      see all

    Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker 图像

    • ab11826 staining SC35 in human fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 5% Normal Goat Serum/0.3% Triton X-100 in PBS for 60 minutes at 25°C. Samples were incubated with primary antibody (1/500 in 1% BSA/ 0.3% Triton X-100 in PBS) for 16 hours at 4°C. An Alexa Flour® 488 goat anti-mouse IgG (H+L), F(ab')2 Fragment Ig was used as the secondary antibody at a dilution of 1/1000.

    • ab11826 (1/1000) staining SC35 (phospho) in Human retinal pigment epithelial (RPE) cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). Please refer to abreview for further experimental details.

      See Abreview

    • ab11826 staining cultured human colon adenocarcinoma HT29 cells.  Cells were PFA fixed and permeabillized in Triton X100 and Saponin prior to blocking with 1% BSA for 1 hour at RT.  The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 4°C.  An Alexa Fluor® 594 conjugated goat anti-mouse IgG3 antibody was used as the secondary.

      See Abreview

    • HeLa cells were fixed 24–48 hours after transfection using 4% paraformaldehyde, permeabilised with 0.2% triton X-100/PBS and probed with ab11826 followed by FITC conjugated secondary antibodies (green). After washing with PBS, slides were mounted using Citifluor and analysed by confocal microscopy. Cells were visualised under a Leica laser scanning confocal microscope equipped with a DM-RXE microscope and an argon-krypton laser.
    • ICC/IF image of ab11826 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab11826 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)参考文献

    This product has been referenced in:
    • Carmona-Mora P  et al. The nuclear localization pattern and interaction partners of GTF2IRD1 demonstrate a role in chromatin regulation. Hum Genet N/A:N/A (2015). ICC/IF . Read more (PubMed: 26275350) »
    • Pawellek A  et al. Identification of small molecule inhibitors of pre-mRNA splicing. J Biol Chem 289:34683-98 (2014). ICC/IF ; Human . Read more (PubMed: 25281741) »

    See all 35 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Hippocampus-Paraffin embedded)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH9
    Permeabilization No
    Specification Hippocampus-Paraffin embedded
    Blocking step Scy-tek Superblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    提交于 Jul 16 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (fibroblast)
    Permeabilization Yes - block contains 0.3% tritonX-100 (1H, RT)
    Specification fibroblast
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Paraformaldehyde

    Abcam user community

    Verified customer

    提交于 Jun 29 2016

    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293 (kidney cell))
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Gradient)
    Loading amount 5 µg
    Specification HEK293 (kidney cell)
    Blocking step Licor Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C

    Abcam user community

    Verified customer

    提交于 Jul 18 2015

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Scytek Superblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Sample Human Cell (HEK293 (kidney))
    Specification HEK293 (kidney)
    Permeabilization No
    Fixative Methanol

    Dr. Sam Nowitzki

    Verified customer

    提交于 Apr 06 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Scytek Superblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Antigen retrieval step Heat mediated
    Sample Human Tissue sections (Hippocampus (Brain))
    Specification Hippocampus (Brain)
    Permeabilization No
    Fixative Formaldehyde

    Dr. Sam Nowitzki

    Verified customer

    提交于 Mar 31 2015

    After contacting the laboratory, the IHC-P protocol in the paper through the link below was used successfully with this antibody.

    Our recommended ISH protocol is given thro...

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    Thank you for contacting us again.

    we will gladly provide a free of charge replacement, however, we need to know which antibody we shall send to you.

    The unwanted cross-reactivity is present in all lots, therefore, we cannot provid...

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    Thank you for your nice reply.

    Unfortunately, it is not only a batch problem, but will happen all the time with this antibody. That's why we removed the application and references for WB. this means unfortunately, that I cannot send you a rep...

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    This antibody works fine for ICC, but we found that in WB it shows a possible cross-reactivity with the SC35-related non-snRNP factor SF2/ASF (p75) and Ad2 proteins. This may explain your unexpected results.

    Perdona por la espera.

    La mejor manera de evitar bandas inespecíficas es asegurar unas condiciones de preparación de la muestra idóneas para el anticuerpo en cuestión. Este anticuerpo reconoce el epítopo en ...

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