概述

  • 产品名称Anti-SC35抗体
    参阅全部 SC35 一抗
  • 描述
    兔多克隆抗体to SC35
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human)

  • 阳性对照
    • HeLa nuclear extract

性能

应用

Our Abpromise guarantee covers the use of ab28428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway.
  • 序列相似性Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻译后修饰Extensively phosphorylated on serine residues in the RS domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 35 kDa antibody
    • arginine/serine-rich 2 antibody
    • PR264 antibody
    • Protein PR264 antibody
    • SC 35 antibody
    • SC-35 antibody
    • SC35 antibody
    • Serine/arginine-rich splicing factor 2 antibody
    • SFRS 2 antibody
    • SFRS2 antibody
    • SFRS2A antibody
    • Splicing component 35 kDa antibody
    • Splicing component antibody
    • Splicing factor antibody
    • Splicing factor arginine/serine rich 2 antibody
    • Splicing factor SC35 antibody
    • Splicing speckle antibody
    • Splicing speckles antibody
    • SR splicing factor 2 antibody
    • SRp30b antibody
    • SRSF2 antibody
    • SRSF2_HUMAN antibody
    see all

Anti-SC35 antibody 图像

  • All lanes : Anti-SC35 antibody (ab28428)

    Lane 1 : recombinant SC35 protein
    Lane 2 : HeLa nuclear lysate


    Predicted band size : 26 kDa
  • IHC image of ab28428 staining in human seminoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28428, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SC35 antibody (ab28428)参考文献

This product has been referenced in:
  • Berulava T  et al. FTO levels affect RNA modification and the transcriptome. Eur J Hum Genet : (2012). Read more (PubMed: 22872099) »
  • Toyota M  et al. Epigenetic silencing of microRNA-34b/c and B-cell translocation gene 4 is associated with CpG island methylation in colorectal cancer. Cancer Res 68:4123-32 (2008). WB ; Human . Read more (PubMed: 18519671) »

See all 2 Publications for this product

Product Wall

Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (Bone marrow cell)
Gel Running Conditions Reduced Denaturing (Any kDMini-PROTEAN TGX Gel)
Loading amount 500000 cells
Specification Bone marrow cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jun 10 2015

Thank you for contacting us.

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has c...

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Thank you for clarifying this for me.

As you have used these products in applications and species listed on the datasheet and as you have attempted extensive troubleshooting and I can see no further advice which may help in this instance, we...

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Thank you for your response.

I do wish to apologize for repeatedly inquiring this further information but I wanted to be clear about the issue you have been having. Based on what you have told me you have previously used the anti-SC35 antibo...

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Thank you for this further information.

I would like to repeat that I am sorry that these products are not working as expected for you. I see that you have used the anti-SC35 antibody at a much higher concentration than we have had to use in...

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Thank you for contacting us.

I am sorry to hear of the issues which you have been facing when using these products. I would like to thank you for including your protocols details with this inquiry. You state that you have use up to 800ng. ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (A549 cells (alveolar basal epithelial cells))
Specification A549 cells (alveolar basal epithelial cells)
Fixative Formaldehyde
Permeabilization Yes - 0.5% Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 24 2011

Thank you for your enquiry. ab28428 is a polyclonal antibody generated from baculovirus-expressed recombinant protein and purified through a protein A column. The size of the protein (SC35) detected from HeLa cell nuclear extract is about 30kDa. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"