• 产品名称Anti-SC35抗体
    参阅全部 SC35 一抗
  • 描述
    兔多克隆抗体to SC35
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Guinea pig, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 170-219 (KSKSSSVSRS RSRSRSRSRS RSPPPVSKRE SKSRSRSKSP PKSPEEEGAV) of Human SC35 (NP_003007).

  • 阳性对照
    • HeLa cell lysate.



Our Abpromise guarantee covers the use of ab116103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • 功能Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway.
  • 序列相似性Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻译后修饰Extensively phosphorylated on serine residues in the RS domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 35 kDa antibody
    • arginine/serine-rich 2 antibody
    • PR264 antibody
    • Protein PR264 antibody
    • SC 35 antibody
    • SC-35 antibody
    • SC35 antibody
    • Serine/arginine-rich splicing factor 2 antibody
    • SFRS 2 antibody
    • SFRS2 antibody
    • SFRS2A antibody
    • Splicing component 35 kDa antibody
    • Splicing component antibody
    • Splicing factor antibody
    • Splicing factor arginine/serine rich 2 antibody
    • Splicing factor SC35 antibody
    • Splicing speckle antibody
    • Splicing speckles antibody
    • SR splicing factor 2 antibody
    • SRp30b antibody
    • SRSF2 antibody
    • SRSF2_HUMAN antibody
    see all

Anti-SC35 antibody 图像

  • Anti-SC35 antibody (ab116103) at 1 µg/ml + HeLa cell lysate at 10 µg

    Predicted band size : 25 kDa

Anti-SC35 antibody (ab116103)参考文献

ab116103 has not yet been referenced specifically in any publications.

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Thank you for submitting an Abreview of ab116103. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a untested species and application (mouse, ICC/IF), we would like t...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH/3T3 mouse embryonic fibroblast)
Specification NIH/3T3 mouse embryonic fibroblast
Fixative Formaldehyde
Permeabilization Yes - PBS with 0.5% Triton-X100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C

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Verified customer

提交于 Jun 01 2012


ab115184: x

ab116103: y

Ablaufdatum jeweils: dd mm 2012

Es freut mich zu hören, dass Sie unser Angebot unsere Antikörper der IF zu testen, annehmen möchten. Wie besprochen...

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