The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May play a role as marker for the diagnosis of metastatic breast cancer.
Expressed in mammary, salivary glands and prostate. Also detected in lung. Mainly expressed in cancer cell lines of breast origin. Highly expressed in lymph node-positive compared with node-negative tumors. Detected in all lymph node containing metastatic cells.
Immunohistochemical staining of SBEM in paraffin embedded Human breast tissue by Immunohistochemistry, using ab122530 at a 1/20 dilution. The image shows moderate cytoplasmic and extracellular positivity in glandular cells.
Liu L et al. Small breast epithelial mucin tumor tissue expression is associated with increased risk of recurrence and death in triple-negative breast cancer patients. Diagn Pathol8:71 (2013).
Read more (PubMed: 23635316) »