Anti-SATB2抗体[SATBA4B10] (ab51502)


  • 产品名称Anti-SATB2抗体[SATBA4B10]
    参阅全部 SATB2 一抗
  • 描述
    小鼠单克隆抗体[SATBA4B10] to SATB2
  • 经测试应用适用于: IP, ICC/IF, IHC-FoFr, WB, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Zebrafish
  • 免疫原

    Recombinant fragment corresponding to Human SATB2 (C terminal).

  • 阳性对照
    • HT-1080 whole cell lysate. RIPA lysateof HeLacells. HeLa cells. NIH3T3 whole cell lysate.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, 8mM Dibasic monohydrogen sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Monobasic dihydrogen potassium phosphate, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明Concentrated and dialyzed culture medium (5% FBS medium without bovine IgG(<95%approximately)) of hybridoma Purified by ProteinG Filtered through a 0.22 µm membrane and packed aseptically.
  • 克隆单克隆
  • 克隆编号SATBA4B10
  • 同种型IgG1
  • 研究领域


Our Abpromise guarantee covers the use of ab51502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP 1/100.
ICC/IF 1/5.
IHC-FoFr Use at an assay dependent concentration.
WB 1/100. Predicted molecular weight: 81 kDa.
IHC-Fr 1/50 - 1/400.
IHC-P 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • 功能Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.
  • 组织特异性High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.
  • 疾病相关Note=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
    Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
    Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22).
  • 序列相似性Belongs to the CUT homeobox family.
    Contains 2 CUT DNA-binding domains.
    Contains 1 homeobox DNA-binding domain.
  • 翻译后修饰Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.
  • 细胞定位Nucleus matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA binding protein SATB2 antibody
    • DNA-binding protein SATB2 antibody
    • FLJ21474 antibody
    • FLJ32076 antibody
    • GLSS antibody
    • KIAA1034 antibody
    • MGC119474 antibody
    • MGC119477 antibody
    • SATB family member 2 antibody
    • SATB homeobox 2 antibody
    • SATB2 antibody
    • SATB2_HUMAN antibody
    • Special AT rich sequence binding protein 2 antibody
    • Special AT-rich sequence-binding protein 2 antibody
    see all

Anti-SATB2 antibody [SATBA4B10] 图像

  • Anti-SATB2 antibody [SATBA4B10] (ab51502) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

    Anti Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 81 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Same results obtained using HT-1080 whole cell lysate at 25µg.
  • ab51502 staining SATB2 (red) in a mouse cortex e17.5 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 3% Donkey serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/10) for 90 minutes at 22°C. An Alexa Fluor®555-conjugated Donkey anti-mouse polyclonal (1/200) was used as the secondary antibody.
    Green: CTIP2 (ab18465) staining
    Blue: TBR1 (ab31940) staining

    See Abreview

  • ab51502 staining SATB2 in human fetal cortex tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer pH 6.0. Samples were then blocked with 10% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/100 dilution for 24 hours at 4°C. A Cy3®-conjugated horse anti-mouse polyclonal was used as secondary antibody at a 1/200 dilution. Counter-stained with DAPI (blue).

    See Abreview

  • ab51502 staining SATB2 in HeLa cells by Immunocytochemistry 1:200.
  • ab51502 staining cultured rat cortical neuron cells by ICC/IF.  Cells were PFA fixed, permeabilized in 0.1% triton and blocked with 10% BSA for 1 hour at 22°C.  The sample was then incubated with ab51502, diluted 1/25 for 2 hours at 22°C prior to incubation with a Cy3® conjugated donkey anti-mouse antibody, diluted 1/400.  In the image, red is SATB2 and green is F-actin

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  • ab51502 staining SATB2 in mouse brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to mouse IgG (1/1000) was used as secondary antibody.

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  • Immunoprecipitation using ab51502 at 1:100 dilution. Sample :NIH3T3 whole cell lysate 25µg. Secondary used at 1:2500.

Anti-SATB2 antibody [SATBA4B10] (ab51502)参考文献

This product has been referenced in:
  • Jara JH  et al. Healthy and diseased corticospinal motor neurons are selectively transduced upon direct AAV2-2 injection into the motor cortex. Gene Ther N/A:N/A (2016). ICC/IF ; Mouse . Read more (PubMed: 26704722) »
  • Leone DP  et al. Compensatory Actions of Ldb Adaptor Proteins During Corticospinal Motor Neuron Differentiation. Cereb Cortex N/A:N/A (2016). Read more (PubMed: 26830346) »

See all 43 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Permeabilization No
Specification Skin
Blocking step Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde

Ahmar Aziz

Verified customer

提交于 Jul 08 2016

Application Immunohistochemistry (Frozen sections)
Blocking step NGS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - Triton-X 0.5%
Fixative Paraformaldehyde

Marcos Romualdo Costa

Verified customer

提交于 Jul 18 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step None
Sample Rat Tissue sections (E9.5 rat placenta)
Specification E9.5 rat placenta
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde

Abcam user community

Verified customer

提交于 Nov 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain 50um section)
Specification Brain 50um section
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization Yes - 0.3% Triton X-100 in blocking step
Blocking step Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

提交于 Jul 31 2012

Of the two rabbit monoclonals, ab92446 (SATB2) and ab92307 (SATB1), ab92446 is more likely to be specific for its target, SATB2, than ab92307 is for SATB1.

Click here (or use the following: <...

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Thank you for sending the catalogue abID's.

The alignments of the immunogens with the other proteins do suggest that these antibodies may not have the specificity you hope for:

The ab92307 immunogen shares 73% identity (11/15) wit...

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Thank you for contacting us. The concentration of ab51502 is 0.1 mg/ml and should be used at a dilution of 1/100 for both IP and WB. Regarding ab92445, this is a tissue culture supernatant antibody so the concentration is not determined. Generally conc...

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Application Immunohistochemistry (Frozen sections)
Sample Zebra finch Tissue sections (embryonal spinal cord)
Specification embryonal spinal cord
Fixative Paraformaldehyde
Permeabilization Yes - 0.1TX100 in PBS
Blocking step Roti-Immunoblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Dr. Robert Storm

Verified customer

提交于 Feb 06 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for bringing this to our attention. After reading your email I was able to perform an alignmet of the immunogen sequence used to create this product against mouse and rat SATB1 and SATB2 proteins. I found that there was62% homolgy between mur...

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