RabMAb

Anti-SATB2抗体[EPNCIR130A] (ab92446)

概述

性能

应用

Our Abpromise guarantee covers the use of ab92446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 81 kDa.
IHC-P 1/150 - 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ICC/IF 1/100.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.
    • 组织特异性High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.
    • 疾病相关Note=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
      Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
      Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22).
    • 序列相似性Belongs to the CUT homeobox family.
      Contains 2 CUT DNA-binding domains.
      Contains 1 homeobox DNA-binding domain.
    • 翻译后修饰Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.
    • 细胞定位Nucleus matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DNA binding protein SATB2 antibody
      • DNA-binding protein SATB2 antibody
      • FLJ21474 antibody
      • FLJ32076 antibody
      • GLSS antibody
      • KIAA1034 antibody
      • MGC119474 antibody
      • MGC119477 antibody
      • SATB family member 2 antibody
      • SATB homeobox 2 antibody
      • SATB2 antibody
      • SATB2_HUMAN antibody
      • Special AT rich sequence binding protein 2 antibody
      • Special AT-rich sequence-binding protein 2 antibody
      see all

    Anti-SATB2 antibody [EPNCIR130A] 图像

    • Unpurified ab92446 staining SATB2 in E18 Mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton-X 100 and blocked with 3% BSA for 30 minutes at 25°C. The sample was incubated with primary antibody (1/500 in TBS with 0.1% Triton-X 100 + 3% Goat serum) at 4°C for 12 hours. An Alexa Fluor® 546-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • Unpurified ab92446 staining SATB2 in mouse brain tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.3% Triton-X-100. Samples were then blocked with 5% serum for 1 hour 30 minutes at 20°C followed by incubation with the primary antibody at a 1/200 dilution for 36 hours. A biotin conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/200 dilution.

      See Abreview

    • Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution (purified) + SW1353 cell lysate at 20 µg

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 81 kDa
      Observed band size : 83 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution (purified)

      Lane 1 : Saos-2 cell lysate
      Lane 2 : Human fetal brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 81 kDa
      Observed band size : 83 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 81 kDa
      Observed band size : 83 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution (unpurified)

      Lane 1 : HT-1080 cell lysate
      Lane 2 : SW1353 cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 81 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling SATB2 with purified ab92446 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling SATB2 (green) with purified ab92446 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

      Control: primary antibody (1/100) and secondary antibody ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Anti-SATB2 antibody [EPNCIR130A] (ab92446)参考文献

    This product has been referenced in:
    • Mao H  et al. Haploinsufficiency for Core Exon Junction Complex Components Disrupts Embryonic Neurogenesis and Causes p53-Mediated Microcephaly. PLoS Genet 12:e1006282 (2016). Read more (PubMed: 27618312) »
    • Wille A  et al. Impaired Contextual Fear Extinction Learning is Associated with Aberrant Regulation of CHD-Type Chromatin Remodeling Factors. Front Behav Neurosci 9:313 (2015). IF ; Mouse . Read more (PubMed: 26635563) »

    See all 6 Publications for this product

    Product Wall

    Thank you for your call yesterday.
    I now heard back form the source of this antibody and I am very happy to confirm that we can provide a range of amino acids where the immunogen in a part of.
    Unfortunately the exact sequence is proprietary a...

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    Application Immunocytochemistry
    Sample Mouse Cultured Cells (cortical neurons)
    Specification cortical neurons
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Apr 12 2016

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Antigen retrieval step None
    Sample Mouse Tissue sections (E18 mouse brain)
    Specification E18 mouse brain
    Permeabilization Yes - 0.3% Triton X-100
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Nov 11 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (MDA-MB231, H1299, SHSY5Y and HEK293T cells)
    Loading amount 30 µg
    Specification MDA-MB231, H1299, SHSY5Y and HEK293T cells
    Gel Running Conditions Reduced Denaturing (10 % PAGE gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 02 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (MDA-MB231 cells)
    Specification MDA-MB231 cells
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1 % TritonX-100 for 5 minutes
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 02 2012

    Thank you for contacting us. The concentration of ab51502 is 0.1 mg/ml and should be used at a dilution of 1/100 for both IP and WB. Regarding ab92445, this is a tissue culture supernatant antibody so the concentration is not determined. Generally conc...

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    Thank you for contacting us. I am very sorry to hear that you are having problems with this antibody. Could you please let me know a few more experimental details (for our records): 1) What sample type was used and what is the species of the samp...

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    Thank you for your email. I am very glad to hear that you obtained the desired results. As for another SATB2 antibody that is suitable for IP/WB, we have also ab92446: http://www.abcam.com/SATB2-antibody-EPNCIR130A-ab92446.html It was tested in I...

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    Thank you for your reply. Unfortunately, the specific immunogen sequence is proprietary and I am unable to disclose this information.  All other available information regarding this product is available on the online datasheet. I hope this info...

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    Thank you for contacting Abcam regarding ab92446. I have checked our catalog and unfortunately we do not have a blocking peptide available for this antibody. I apologize for any inconvenience this may cause.  Please do not hesitate to contact u...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"