概述

  • 产品名称Anti-SATB2抗体
    参阅全部 SATB2 一抗
  • 描述
    兔多克隆抗体to SATB2
  • 特异性ab69998 does not cross-react with SATB1.
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic 14 amino acid peptide near the carboxy terminus of the human SATB2 (GenBank accession no. Q9UPW6).

  • 阳性对照
    • A20 cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab69998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 82, 65 kDa (predicted molecular weight: 82 kDa).

靶标

  • 功能Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.
  • 组织特异性High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.
  • 疾病相关Note=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
    Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
    Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22).
  • 序列相似性Belongs to the CUT homeobox family.
    Contains 2 CUT DNA-binding domains.
    Contains 1 homeobox DNA-binding domain.
  • 翻译后修饰Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.
  • 细胞定位Nucleus matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA binding protein SATB2 antibody
    • DNA-binding protein SATB2 antibody
    • FLJ21474 antibody
    • FLJ32076 antibody
    • GLSS antibody
    • KIAA1034 antibody
    • MGC119474 antibody
    • MGC119477 antibody
    • SATB family member 2 antibody
    • SATB homeobox 2 antibody
    • SATB2 antibody
    • SATB2_HUMAN antibody
    • Special AT rich sequence binding protein 2 antibody
    • Special AT-rich sequence-binding protein 2 antibody
    see all

Anti-SATB2 antibody 图像

  • Lane 1 : Anti-SATB2 antibody (ab69998) at 2 µg/ml
    Lane 2 : Anti-SATB2 antibody (ab69998) at 4 µg/ml

    Lane 1 : A20 cell lysate
    Lane 2 : A20 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 82 kDa
    Observed band size : 65,82 kDa (why is the actual band size different from the predicted?)
  • ab69998 at 5 µg/ml staining SATB2 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Immunofluorescence of SATB2 in Human Brain cells using ab69998 at 50 ug/ml.

Anti-SATB2 antibody (ab69998)参考文献

ab69998 has not yet been referenced specifically in any publications.

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