概述

  • 产品名称Anti-SATB1抗体
    参阅全部 SATB1 一抗
  • 描述
    山羊多克隆抗体to SATB1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    C-ESDEENRQKTRPRT

    , corresponding to internal sequence amino acids 636-649 of Human SATB1 (NP_002962.1).

  • 阳性对照
    • MOLT4 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab106941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 86, 110 kDa (predicted molecular weight: 86 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly upregulating metastasis-associated genes while downregulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.
  • 组织特异性Expressed predominantly in thymus.
  • 序列相似性Belongs to the CUT homeobox family.
    Contains 2 CUT DNA-binding domains.
    Contains 1 homeobox DNA-binding domain.
  • 翻译后修饰Sumoylated. Sumoylation promotes cleavage by caspases.
    Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation.
    Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms can not dimerize and lose transcription regulation function because of impaired DNA and chromatin association.
  • 细胞定位Nucleus matrix. Nucleus > PML body. Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies.
  • Information by UniProt
  • 数据库链接
  • 形式There are 2 isoforms produced by alternative splicing.
  • 别名
    • DNA binding protein SATB1 antibody
    • DNA-binding protein SATB1 antibody
    • SATB homeobox 1 antibody
    • SATB1 antibody
    • SATB1_HUMAN antibody
    • Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA) antibody
    • Special AT rich sequence binding protein 1 antibody
    • Special AT-rich sequence-binding protein 1 antibody
    see all

Anti-SATB1 antibody 图像

  • Anti-SATB1 antibody (ab106941) at 0.3 µg/ml + MOLT4 cell lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 86 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 hour
  • ab106941 (3.8µg/ml) staining of paraffin embedded Human Thymus shows select nuclear staining. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-SATB1 antibody (ab106941)参考文献

ab106941 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab106941.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"