Anti-SART3抗体(ab90871)

概述

  • 产品名称Anti-SART3抗体
    参阅全部 SART3 一抗
  • 描述
    兔多克隆抗体to SART3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae
  • 免疫原

    Synthetic peptide within residues: KDLRLVTNRA GKPKGLAYVE YENESQASQA VMKMDGMTIK ENIIKVAISN, corresponding to internal sequence amino acids 828-877 of Human SART3 (NP_055521).

  • 阳性对照
    • Human fetal small intestine lysate.

性能

应用

Our Abpromise guarantee covers the use of ab90871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 110 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Regulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication.
  • 组织特异性Ubiquitously expressed.
  • 疾病相关Defects in SART3 are the cause of disseminated superficial actinic porokeratosis type 1 (DSAP1) [MIM:175900]. DSAP1 is an autosomal dominant disorder, characterized by multiple superficial keratotic lesions surrounded by a slightly raised keratotic border, developing during the third or fourth decade of life on sun-exposed areas of skin.
  • 序列相似性Contains 8 HAT repeats.
    Contains 2 RRM (RNA recognition motif) domains.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm. Nucleus speckle. Localized in speckles. Expressed in the nucleus of all of the malignant tumor cell lines tested and the majority of cancer tissues with various histologies, including squamous cell carcinomas (SCC), adenocarcinomas, melanomas and leukemias cells. However, this protein is undetectable in the nucleus of any cell lines of nonmalignant cells or normal tissues, except for the testis. Expressed in the cytoplasm of all the proliferating cells, including normal and malignant cells, but not in normal tissues, except for the testis and the fetal liver.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DSAP1 antibody
    • hSART 3 antibody
    • hSART-3 antibody
    • P100 antibody
    • P110 antibody
    • p110(nrb) antibody
    • RP11 13G14 antibody
    • SART 3 antibody
    • SART-3 antibody
    • SART3 antibody
    • SART3_HUMAN antibody
    • Squamous cell carcinoma antigen recognized by T cells 3 antibody
    • Squamous cell carcinoma antigen recognized by T-cells 3 antibody
    • Tat interacting protein of 110 kDa antibody
    • Tat-interacting protein of 110 kDa antibody
    • Tip110 antibody
    see all

Anti-SART3 antibody 图像

  • Anti-SART3 antibody (ab90871) at 1 µg/ml + Human fetal small intestine lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 110 kDa

Anti-SART3 antibody (ab90871)参考文献

ab90871 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Gel Running Conditions Non-reduced Denaturing (12% SDS-PAGE)
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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提交于 Sep 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"