Anti-SARS2抗体- Aminoterminal end (ab71300)

概述

  • 产品名称Anti-SARS2抗体- Aminoterminal end
    参阅全部 SARS2 一抗
  • 描述
    兔多克隆抗体to SARS2 - Aminoterminal end
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide from the N terminus of human SARS2 conjugated to KLH

  • 阳性对照
    • 293 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Ammonium Sulphate Precipitation
  • 纯化说明This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab71300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/50 - 1/100. Detects a band of approximately 68 kDa (predicted molecular weight: 58 kDa).
IHC-P 1/10 - 1/50.

靶标

  • 功能Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).
  • 通路Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.
  • 序列相似性Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.
  • 结构域Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • mitochondrial antibody
    • mtSerRS antibody
    • SARS antibody
    • SARS2 antibody
    • SARSM antibody
    • Serine tRNA ligase antibody
    • Serine--tRNA ligase antibody
    • SerRS antibody
    • SerRSmt antibody
    • SERS antibody
    • Seryl tRNA synthetase 2 antibody
    • Seryl-tRNA synthetase antibody
    • Seryl-tRNA(Ser/Sec) synthetase antibody
    • SYS antibody
    • SYSM_HUMAN antibody
    see all

Anti-SARS2 antibody - Aminoterminal end 图像

  • Anti-SARS2 antibody - Aminoterminal end (ab71300) at 1/60 dilution + 293 cell lysate at 35 µg

    Predicted band size : 58 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 54 kDa,72 kDa. We are unsure as to the identity of these extra bands.
  • ab71300 at 1/50 dilution staining SARS2 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

Anti-SARS2 antibody - Aminoterminal end (ab71300)参考文献

ab71300 has not yet been referenced specifically in any publications.

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