The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 139 kDa (predicted molecular weight: 139 kDa).
A novel coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive stranded RNA approximately 27 to 31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.
E2 glycoprotein antibody
Human coronavirus spike glycoprotein antibody
S glycoprotein antibody
Spike glycoprotein antibody
Western blot - Anti-SARS spike glycoprotein antibody (ab71101)
All lanes : Anti-SARS spike glycoprotein antibody (ab71101) at 1/1000 dilution
Lane 1 : Recombinant SARS spike glycoprotein (aa 17-537) Lane 2 : Recombinant SARS spike glycoprotein (aa 17-756)
Predicted band size: 139 kDa Observed band size: 139 kDa