The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ELISA: Use at a concentration of 0.1-1.0 µg/ml.
WB: Use at a concentration of 0.1-1.0 µg/ml. Detects a band of approximately 139 kDa.
[The antibody was tested on a cell line transfected with a partial-length cDNA coding for the N-terminal portion of SARS Spike protein with a predicted molecular weight of 68 kDa.]
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
相关性A novel coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive stranded RNA approximately 27 to 31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.
E2 glycoprotein antibody
Human coronavirus spike glycoprotein antibody
S glycoprotein antibody
Spike glycoprotein antibody
Anti-SARS spike glycoprotein antibody 图像
Western blot - SARS spike glycoprotein antibody (ab22159)
All lanes : Anti-SARS spike glycoprotein antibody (ab22159) at 0.1 µg/ml
Lane 1 : Transfected mouse melanoma cell lysate. Lane 2 : Untransfected mouse melanoma cell lysate.