概述

  • 产品名称
    Anti-SARS Nsp2抗体
  • 描述
    小鼠多克隆抗体to SARS Nsp2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    Reacts with SARS Coronavirus. Not yet tested in other species.
  • 免疫原

    Fusion protein:

    SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDTVYCPRHVICTAEDML NPNYEDLLIRKSNHSFLVQAGNVQLRVIGHSMQNCLLRLKVDTSNPKTPK Y

    , corresponding to amino acids 1-101 of SARS Nsp2.

  • 常规说明
    Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Constituents: 50% Glycerol
  • 纯度
    Whole antiserum
  • Primary antibody说明
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab21657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 37 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 相关性
    The main proteinase Nsp2 is responsible for the majority of cleavages as it cleaves the C terminus of replicase polyprotein at 11 sites. It recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. It is inhibited by the substrate analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
  • 别名
    • 3C like proteinase antibody
    • 3CL PRO antibody
    • 3CLp antibody

图片

  • All lanes : Anti-SARS Nsp2 antibody (ab21657) at 1/1000 dilution

    Lane 1 : 20ug of a total protein extract from E. coli with ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen.
    Lane 2 : 20ug of a total protein extract from E. coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein).

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 5000

    Performed under reducing conditions.

    Predicted band size : 37 kDa

实验方案

文献

ab21657 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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