概述

  • 产品名称
  • 描述
    兔多克隆抗体to SARM
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) (C terminal).

  • 阳性对照
    • Daudi cells

性能

应用

Our Abpromise guarantee covers the use of ab17812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 80 kDa.
ICC/IF Use a concentration of 2 µg/ml.

靶标

  • 功能
    Involved in innate immnune response. Acts as a negative regulator of TICAM1/TRIF-dependent Toll-like receptor signaling by inhibiting induction of TLR3- and TLR4-dependent genes. Specifically blocks TICAM1/TRIF-dependent transcription-factor activation and gene induction, without affecting the MYD88-dependent pathway or non-TLR signaling. Negative regulator of NF-kappa-B and IRF activation.
  • 组织特异性
    Predominantly expressed in kidney and liver. Expressed at lower level in placenta.
  • 序列相似性
    Contains 2 SAM (sterile alpha motif) domains.
    Contains 1 TIR domain.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ36296 antibody
    • KIAA0524 antibody
    • MyD88-5 antibody
    • SAM and ARM-containing protein antibody
    • SAM domain-containing protein 2 antibody
    • SAMD2 antibody
    • SARM 1 antibody
    • SARM1 antibody
    • SARM1_HUMAN antibody
    • Sterile alpha and Armadillo repeat protein antibody
    • sterile alpha and HEAT/Armadillo motif protein, ortholog of Drosophila antibody
    • sterile alpha and HEAT/Armadillo motifs-containing protein antibody
    • sterile alpha and TIR motif containing 1 antibody
    • Sterile alpha and TIR motif-containing protein 1 antibody
    • Sterile alpha and TIR motifs-containing protein 1 antibody
    • Sterile alpha motif domain-containing protein 2 antibody
    • Tir-1 homolog antibody
    see all

图片



  • Predicted band size : 80 kDa
    Western Blot of Daudi cell lysate labeling SARM with Anti-SARM antibody (ab17812) at (1) 0.5 and (2) 1µg/ml.
  • Lane 1 : Anti-SARM antibody (ab17812) at 0.5 µg/ml
    Lane 2 : Anti-SARM antibody (ab17812) at 1 µg/ml
    Lane 3 : Anti-SARM antibody (ab17812) at 2 µg/ml

    Lane 1 : Daudi cell lysate
    Lane 2 : Daudi cell lysate
    Lane 3 : Daudi cell lysate


    Predicted band size : 80 kDa
    Observed band size : 80 kDa
    Western blot analysis of SARM (79 kDa) in Daudi cell lysates with ab17812 at (A) 0.5, (B) 1, and (C) 2 µg/ml.
  • ab17812 at 2µg/ml staining of SARM in Daudi cells by ICC/IF 

文献

This product has been referenced in:
  • de Vos AF  et al. In vivo lipopolysaccharide exposure of human blood leukocytes induces cross-tolerance to multiple TLR ligands. J Immunol 183:533-42 (2009). WB ; Human . Read more (PubMed: 19542464) »

See 1 Publication for this product

客户评价及客户问答

Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (7.5% gel)
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Fly head)
Specification
Fly head
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Feb 10 2015

Thank you for contacting us. We do not know what the identity of the additional bands on the WB image are. They may be non-specific bands. The immunogen used to generate the antibody is chosen to be unique, so that it is unlikely to react with other pr...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (RAJI B-Cells)
Loading amount
20 µg
Specification
RAJI B-Cells
Gel Running Conditions
Reduced Denaturing (12% Tris-HEPES-SDS Gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Aug 14 2009

Thank you for your enquiry. I have been informed that unfortunately the homology is only 61%, therefore we predict that there is little chance for cross reaction with zebrafish protein; there are 6 highly conserved residues next to each other, enoug...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (monocytes)
Loading amount
20 µg
Specification
monocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Feb 19 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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