Sarcosine Assay试剂盒(ab65338)

概述

  • 产品名称Sarcosine Assay试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Cell culture supernatant, Milk, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型Quantitative
  • 灵敏度
    > 1 µM
  • 范围
    1 µM - 10000 µM
  • 检测时间
    1h 00m
  • 产品概述

    Abcam's Sarcosine Assay Kit provides an accurate, convenient measure of sarcosine in variety biological samples. In the assay, sarcosine is specifically oxidized to generate a product that converts a colorless probe to a product with intense red color (?max = 570 nm) and which is also highly fluorescent (Ex/Em = 538/587 nm). Sarcosine is therefore easily detected by either colorimetric or fluorometric methods with detection range 1-10000 µM.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Sarcosine, a natural amino acid, plays important roles as intermediate in the metabolism of choline, methionine, glycine, glutathione, creatine, purine and serine, etc. Detection of sarcosine level has wide applications in research and development.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Sarcosine (N-methylglycine) is an intermediate and byproduct in the synthesis and degradation of glycine. Sarcosine is metabolized to glycine by sarcosine dehydrogenase, while glycine-N-methyl transferase generates sarcosine from glycine. Sarcosine is a natural amino acid found in muscles and other body tissues and plays important role as an intermediate in the metabolism of choline, methionine, glycine, glutathione, creatine, purine and serine.
  • 别名
    • N methylglycine

应用

Our Abpromise guarantee covers the use of ab65338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies
  • 应用说明FuncS: Use at an assay dependent dilution.
    Note: Urine samples do not work well with the assay due to sample interferences.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Sarcosine Assay Kit 图像

    • Sarcosine Standard Curve performed using ab65338.

    实验方案

    Sarcosine Assay Kit (ab65338)参考文献

    ab65338 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. We have contacted our source to request details of the composition of the assay buffer, enzyme mix and probe and I am sorry they have regrettably informed me that this is commercially sensitive information and they a...

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    Thank you for your enquiry. Please find below the answers to your questions. 1. Biospecimens like body fluids (plasma/ serum), tissues, and cell culture samples can be used. However, urine samples will give interference with the assay. For c...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"