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Full length native protein (purified) corresponding to Rabbit Sarcomeric Alpha Actinin. Purified rabbit skeletal α-actinin.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab68167 as a replacement.
Our Abpromise guarantee covers the use of ab9465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/50. ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17383364|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20019242|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue staining Sarcomeric Alpha Actinin with ab9465.
Immunocytochemistry/Immunofluorescence analysis of H9 hESC and CBiPSC6.2 cells labelling Sarcomeric Alpha Actinin (green) with ab9465 at a dilution of 1/200. An Alexa Fluor® 488-conjugated goat anti-mouse IgG was used as the secondary antibody (1/200).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"