The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 180 kDa (predicted molecular weight: 156 kDa).
1/10 - 1/100.
1/100 - 1/250.
1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
应用说明Is unsuitable for ICC.
功能Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization.
组织特异性Ubiquitous. In the brain found primarily in the cerebrovascular smooth muscle cells and reactive astrocytes.
序列相似性Contains 1 FYVE-type zinc finger.
结构域The SMAD binding domain (SBD) interacts with the MH2 domains of SMAD2 or SMAD3. The FYVE-type zinc finger is necessary and sufficient for its localization into early endosomes and mediates the association with PI3P.