Anti-SARA抗体[EPR5020] (ab124875)


  • 产品名称Anti-SARA抗体[EPR5020]
    参阅全部 SARA 一抗
  • 描述
    兔单克隆抗体[EPR5020] to SARA
  • 经测试应用适用于: WB, IP, IHC-P, Flow Cytmore details
    不适用于: ICC
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues at the N-terminus of Human SARA.

  • 阳性对照
    • Huan heart, A673, HT-1080, BxPC-3, Jutkat, HeLa, and Human fetal brain lysates, Human kidney tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab124875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 180 kDa (predicted molecular weight: 156 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC.
  • 靶标

    • 功能Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization.
    • 组织特异性Ubiquitous. In the brain found primarily in the cerebrovascular smooth muscle cells and reactive astrocytes.
    • 序列相似性Contains 1 FYVE-type zinc finger.
    • 结构域The SMAD binding domain (SBD) interacts with the MH2 domains of SMAD2 or SMAD3.
      The FYVE-type zinc finger is necessary and sufficient for its localization into early endosomes and mediates the association with PI3P.
    • 细胞定位Cytoplasm. Early endosome membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CG15667 antibody
      • hSARA antibody
      • LD33044p antibody
      • Madh-interacting protein antibody
      • MADHIP antibody
      • Mothers against decapentaplegic homolog-interacting protein antibody
      • Novel serine protease antibody
      • NSP antibody
      • Receptor activation anchor antibody
      • SAR1 antibody
      • SARA antibody
      • SARA1 antibody
      • Smad anchor for receptor activation antibody
      • SMADIP antibody
      • ZFYV9_HUMAN antibody
      • ZFYVE9 antibody
      • Zinc finger FYVE domain-containing protein 9 antibody
      see all

    Anti-SARA antibody [EPR5020] 图像

    • All lanes : Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : A673 cell lysate
      Lane 3 : HT-1080 cell lysate
      Lane 4 : BxPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 156 kDa
    • All lanes : Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 156 kDa
    • Staining of SARA in Paraffin-embedded Human kidney tissue by Immunohistochemistry using ab124875 at a dilution of 1/100.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-SARA antibody [EPR5020] (ab124875)参考文献

    ab124875 has not yet been referenced specifically in any publications.

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