WB, IP more details
Synthetic peptide from a region between residue 743 and the C-terminus
(residue 793) of human SAPS3,( NP_060782.2).
Whole cell lysate from Hela cells.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 98 kDa).
Use at 2-5 µg/mg of lysate.
功能 Regulatory subunit of protein phospatase 6 (PP6). May function as a scaffolding PP6 subunit. May have an important role in maintaining immune self-tolerance.
组织特异性 Expressed in skeletal muscle, placenta, heart, pancreas, testis, brain, lung, liver, kidney, spleen, thymus, prostate, small intestine, colon and leukocytes.
序列相似性 Belongs to the SAPS family.
翻译后修饰 Phosphorylated upon DNA damage, probably by ATM or ATR.
细胞定位 Cytoplasm. Nucleus.
Information by UniProt
Chromosome 11 open reading frame 23 antibody
Anti-SAPS3 antibody 图像
Western blot - SAPS3 antibody (ab72034)
All lanes : Anti-SAPS3 antibody (ab72034) at 0.02 µg/ml Lane 1 : Whole cell lysate from Hela cells at 50 µg Lane 2 : Whole cell lysate from Hela cells at 15 µg Lane 3 : Whole cell lysate from Hela cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg Predicted band size : 98 kDa Observed band size : 120 kDa ( why is the actual band size different from the predicted?)
Immunoprecipitation - SAPS3 antibody (ab72034)
Immunoprecipitation/ Western Blot of SAPS3.
Lane 1: For IP: ab72034 at 3µg/mg whole cell lysate, with ab72034 at 0.04µg/ml for subsequent WB.
Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
Anti-SAPS3 antibody (ab72034)参考文献
has not yet been referenced specifically in any publications.
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