The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 105 kDa).
Use at 2-5 µg/mg of lysate.
SAPS2 is a regulatory subunit for the protein phosphatase-6 catalytic subunit (PPP6C). Protein phosphatase regulatory subunits, such as SAPS2, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. It is ubiquitously expressed with strongest expression in the testis followed by liver, heart, kidney, brain and placenta. There are two named isoforms.
All lanes : Anti-SAPS2 antibody (ab72032) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg Lane 2 : Whole cell lysate from Hela cells at 15 µg Lane 3 : Whole cell lysate from Hela cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg