概述

  • 产品名称Anti-SAPS2抗体
    参阅全部 SAPS2 一抗
  • 描述
    兔多克隆抗体to SAPS2
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide from a region between residue 775 and 825 of human SAPS2, NP_055493.2.

  • 阳性对照
    • Whole cell lysate from Hela and 293T cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects bands of approximately 130 and 150 kDa (predicted molecular weight: 105 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性SAPS2 is a regulatory subunit for the protein phosphatase-6 catalytic subunit (PPP6C). Protein phosphatase regulatory subunits, such as SAPS2, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. It is ubiquitously expressed with strongest expression in the testis followed by liver, heart, kidney, brain and placenta. There are two named isoforms.
    • 细胞定位Cytoplasm
    • 数据库链接
    • 别名
      • SAPS 2 antibody
      • dJ579N16.1 antibody
      • KIAA0685 antibody
      • PP6R2 antibody
      • SAP190 antibody
      • SAPS domain family, member 2 antibody
      see all

    Anti-SAPS2 antibody 图像

    • All lanes : Anti-SAPS2 antibody (ab72031) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg


      Predicted band size : 105 kDa
      Observed band size : 130 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.
    • Immunoprecipitation/ Western Blot of SAPS2.
      Lane 1: ab72031 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      ab72031 at 1 µg/ml was used for the Western blot detection step.
      Chemiluminescence with an exposure time of 3 seconds.

    Anti-SAPS2 antibody (ab72031)参考文献

    ab72031 has not yet been referenced specifically in any publications.

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