概述

  • 产品名称
  • 描述
    兔多克隆抗体to SAPS1
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide from a region between residue 841 and the C-terminus (residue 891) of human SAPS1, NP_055746.2.

  • 阳性对照
    • Whole cell lysate from Hela cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 103 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 相关性
    Protein phosphatase regulatory subunits, such as SAPS1, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. SAPS1 is a regulatory subunit for the protein phosphatase-6 catalytic subunit. It is ubiquitous, with higher expression in testis. There are two named isoforms.
  • 细胞定位
    Cytoplasm
  • 数据库链接
  • 别名
    • KIAA1115 antibody
    • PP6R1 antibody
    • Protein phosphatase 6, regulatory subunit 1 antibody
    • SAP190 antibody
    • SAPS domain family, member 1 antibody
    see all

图片

  • All lanes : Anti-SAPS1 antibody (ab72030) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg


    Predicted band size : 103 kDa
    Observed band size : 117 kDa (why is the actual band size different from the predicted?)
  • Immunoprecipitation/ Western Blot of SAPS1.
    Lane 1: ab72030 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    ab72030 at 0.1 µg/ml was used for the western blot detection step. Chemiluminescence with an exposure time of 3 seconds.

文献

ab72030 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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