Anti-SAP97抗体(ab60551)

概述

  • 产品名称Anti-SAP97抗体
    参阅全部 SAP97 一抗
  • 描述
    兔多克隆抗体to SAP97
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Human, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Mouse SAP97.

  • 阳性对照
    • This antibody gave a positive signal in Mouse Thymus Tissue Lysate, as well as NIH3T3 and MEF1 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab60551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.
  • 组织特异性Abundantly expressed in atrial myocardium (at protein level). Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level). Widely expressed, with isoforms displaying different expression profiles.
  • 序列相似性Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 1 L27 domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • 结构域The alternatively spliced domain I3 corresponding to amino acids (636-669) of isoform 4 is an EPB41 binding site mediating association to membranes in polarized and non-polarized cells.
    The PDZ domains may also mediate association to membranes by binding to EPB41 and ADGRA2 together with the L27 domain that binds CASK and DLG2.
    The L27 domain may regulate DLG1 self-association. The N-terminal alternatively spliced region is capable of binding several SH3 domains and also moderates the level of protein oligomerization.
  • 翻译后修饰Phosphorylated by MAPK12. Phosphorylation of Ser-232 regulates association with GRIN2A.
  • 细胞定位Membrane. Basolateral cell membrane. Endoplasmic reticulum membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Cell membrane, sarcolemma. Colocalizes with EPB41 at regions of intercellular contacts. Basolateral in epithelial cells. May also associate with endoplasmic reticulum membranes. Mainly found in neurons soma, moderately found at postsynaptic densities (By similarity).
  • Information by UniProt
  • 数据库链接
  • 别名
    • Discs large homolog 1 antibody
    • discs large, Drosophila, homolog of, 1 antibody
    • discs, large homolog 1 (Drosophila) antibody
    • Disks large homolog 1 antibody
    • dJ1061C18.1.1 antibody
    • DKFZp761P0818 antibody
    • DKFZp781B0426 antibody
    • DLG 1 antibody
    • DLG1 antibody
    • DLG1_HUMAN antibody
    • DLGH 1 antibody
    • DLGH1 antibody
    • hDlg antibody
    • Presynaptic protein SAP97 antibody
    • SAP 97 antibody
    • SAP-97 antibody
    • SAP97 antibody
    • Synapse associated protein 97 antibody
    • Synapse-associated protein 97 antibody
    see all

Anti-SAP97 antibody 图像

  • All lanes : Anti-SAP97 antibody (ab60551) at 1 µg/ml

    Lane 1 : Thymus (Mouse) Tissue Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 100 kDa
    Observed band size : 100 kDa
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ICC/IF image of ab60551 stained MEF1 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60551, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MEF1 cells at 5µg/ml.

Anti-SAP97 antibody (ab60551)参考文献

ab60551 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Sample Mouse Cell (Lymphocytes)
Specification Lymphocytes
Gating Strategy CD8+ T cells
Permeabilization Yes - 0.1% Saponin
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提交于 Oct 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"