Anti-SAP30抗体(ab56527)

概述

  • 产品名称Anti-SAP30抗体
    参阅全部 SAP30 一抗
  • 描述
    小鼠单克隆抗体to SAP30
  • 经测试应用适用于: IHC-P, ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: SDDDGGDSPV QDIDTPEVDL YQLQVNTLRR YKRHFKLPTR PGLNKAQLVE IVGCHFRSIP VNEKDTLTYF IYSVKNDKNK SDLKVDSGV, corresponding to amino acids 131-220 of Human SAP30

性能

应用

Our Abpromise guarantee covers the use of ab56527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration. Detection limit for recombinant GST tagged SAP30 is approximately 1ng/ml.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

靶标

  • 功能Involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones.
  • 组织特异性Expressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus; lowest levels in brain, placenta, lung and kidney.
  • 序列相似性Belongs to the SAP30 family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 30 kDa Sin3-associated polypeptide antibody
    • Histone deacetylase complex subunit SAP 30 antibody
    • Histone deacetylase complex subunit SAP30 antibody
    • MSin3A associated polypeptide p30 antibody
    • SAP 30 antibody
    • Sap30 antibody
    • SAP30_HUMAN antibody
    • Sin3 associated polypeptide 30 kDa antibody
    • Sin3 associated polypeptide 30kD antibody
    • Sin3 associated polypeptide antibody
    • Sin3 corepressor complex subunit SAP 30 antibody
    • Sin3 corepressor complex subunit SAP30 antibody
    • Sin3-associated polypeptide p30 antibody
    • Sin3A associated protein 30kDa antibody
    • Sin3A associated protein antibody
    see all

Anti-SAP30 antibody 图像

  • Western blot against tagged recombinant protein immunogen using ab56527 SAP30 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
  • Immunoperoxidase of monoclonal antibody to SAP30 on formalin-fixed paraffin-embedded Human esophagus. [antibody concentration 1.5 ug/ml]
  • Immunofluorescence of monoclonal antibody to SAP30 on HeLa cell . [antibody concentration 10 ug/ml]

Anti-SAP30 antibody (ab56527)参考文献

ab56527 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - other (MCF10A & MCF10CAa.1)
Loading amount 20 µg
Specification MCF10A & MCF10CAa.1
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

提交于 Apr 17 2012

I am very pleased to hear you would like to accept our offer and test ab56527 in endogenous human samples in WB.This code will give you 1 free primary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for endogenous hu...

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