Anti-SAP30抗体(ab125187)

概述

  • 产品名称
    Anti-SAP30抗体
  • 描述
    兔多克隆抗体to SAP30
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Horse, Dog, Pig, Chimpanzee, Gorilla, Chinese hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 170-220 of Human SAP30 (NP_003855.1).

  • 阳性对照
    • Hela, Jurkat, NIH 3T3 and 293T whole cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab125187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 23 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 功能
    Involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones.
  • 组织特异性
    Expressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus; lowest levels in brain, placenta, lung and kidney.
  • 序列相似性
    Belongs to the SAP30 family.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 30 kDa Sin3-associated polypeptide antibody
    • Histone deacetylase complex subunit SAP 30 antibody
    • Histone deacetylase complex subunit SAP30 antibody
    • MSin3A associated polypeptide p30 antibody
    • SAP 30 antibody
    • Sap30 antibody
    • SAP30_HUMAN antibody
    • Sin3 associated polypeptide 30 kDa antibody
    • Sin3 associated polypeptide 30kD antibody
    • Sin3 associated polypeptide antibody
    • Sin3 corepressor complex subunit SAP 30 antibody
    • Sin3 corepressor complex subunit SAP30 antibody
    • Sin3-associated polypeptide p30 antibody
    • Sin3A associated protein 30kDa antibody
    • Sin3A associated protein antibody
    see all

图片

  • All lanes : Anti-SAP30 antibody (ab125187) at 0.4 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 23 kDa


    Exposure time : 30 seconds
  • ab125187, at 6 µg/mg lysate, detecting SAP30 in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
    For the subsequent Western blot, ab125187 was used at 1 µg/ml.
    The blot was developed using the ECL technique, with an exposure time of 10 seconds.

文献

ab125187 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Zebra finch Tissue lysate - whole (cultured embryonic fibroblasts, whole embryo head)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
100000 cells
Specification
cultured embryonic fibroblasts, whole embryo head
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 May 23 2016

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