Involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones.
Expressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus; lowest levels in brain, placenta, lung and kidney.
All lanes : Anti-SAP30 antibody (ab125187) at 0.4 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg Lane 2 : Hela whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
ab125187, at 6 µg/mg lysate, detecting SAP30 in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
For the subsequent Western blot, ab125187 was used at 1 µg/ml.
The blot was developed using the ECL technique, with an exposure time of 10 seconds.