概述

  • 产品名称Anti-SAP155抗体
    参阅全部 SAP155 一抗
  • 描述
    兔多克隆抗体to SAP155
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human SAP155.

    (Peptide available as ab39577)

  • 阳性对照
    • ab39578 gave a positive result in the following lysates: Hela, Jurkat and Jurkat Nuclear. This antibody gave a positive result in IHC in the following FFPE tissue: Human pancreatic adenocarcinoma.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab39578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
  • 序列相似性Belongs to the SF3B1 family.
    Contains 11 HEAT repeats.
  • 翻译后修饰Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis.
  • 细胞定位Nucleus speckle. During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Hsh155 antibody
    • OTTHUMP00000205700 antibody
    • OTTHUMP00000205702 antibody
    • OTTHUMP00000225001 antibody
    • OTTHUMP00000225002 antibody
    • Pre mRNA processing 10 antibody
    • Pre mRNA splicing factor SF3b, 155 kDa subunit antibody
    • Pre-mRNA splicing factor SF3b 155 kDa subunit antibody
    • Pre-mRNA-splicing factor SF3b 155 kDa subunit antibody
    • PRP10 antibody
    • PRPF10 antibody
    • SAP 155 antibody
    • SAP155 antibody
    • sf3b1 antibody
    • SF3B1_HUMAN antibody
    • SF3b155 antibody
    • Spliceosome associated protein 155 antibody
    • Spliceosome-associated protein 155 antibody
    • Splicing factor 3B subunit 1 antibody
    • Splicing factor 3b, subunit 1, 155kDa antibody
    see all

Anti-SAP155 antibody 图像

  • All lanes : Anti-SAP155 antibody (ab39578) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Jurkat nuclear extract lysate (ab14844)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 150 kDa
    Observed band size : 150 kDa
  • ICC/IF image of ab39578 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39578, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • IHC image of SAP155 staining in Human pancreatic adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39578, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SAP155 antibody (ab39578)参考文献

This product has been referenced in:
  • Llorian M  et al. The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators. Nucleic Acids Res 44:8933-8950 (2016). WB ; Mouse . Read more (PubMed: 27317697) »
  • Maserati M  et al. Identification of four genes required for mammalian blastocyst formation. Zygote 22:331-9 (2014). IHC-P ; Mouse . Read more (PubMed: 23211737) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.  The primary UniProt entry for this product is O75533 (this entry is reviewed).  The other two UniProt entries refer to fragments of the same protein.  We expect SAP155 to run at 146 kDa, but is possible that an additio...

Read More

Thank you for your reply and for sending the customer's enquiry.

It may be possible to treat the lysate with a more concentrated CIP solution, however we have found that the recommended concentration tends to give the best results. I searched...

Read More

Thank you for contacting us with your customer's questions. I'm hoping that I can provide some helpful responses.

1) Regarding the lysate treatment, I think it would be fine to freeze the samples prior to CIP treatment. Just lyse the cells as...

Read More

Thanks for getting back to me, and I'm very glad to hear that the customer was satisfied with the previous suggestions.

Every phosphatase will phosphorylate specific amino acids in proteins, and PP1 and PP2A are specific serine/threonine phos...

Read More

Thank you for your reply. My colleague Jackie is out of the office but I am happy to help you with this case. Unfortunately we have not done any further characterization of the bands that appear in the Jurkat nuclear lysate on our website. Ther...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"