概述

  • 产品名称
  • 描述
    兔多克隆抗体to SAP130
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Chicken
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 771 and 1005 of Human SAP130 (UniProt ID: Q9H0E3).

  • 阳性对照
    • MCF7 whole cell lysate; NT2D1, IMR32 and U87-MG cell lysates; PC14 xenograft; MCF7 cells

性能

应用

Our Abpromise guarantee covers the use of ab111739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 110 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (or Tris-EDTA buffer, pH 8.0).
ICC/IF 1/100 - 1/500.

靶标

  • 功能
    Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
  • 组织特异性
    Expressed in various cancer cell ines.
  • 序列相似性
    Belongs to the SAP130 family.
  • 结构域
    The N-terminus may interact with a transcriptional coactivator.
    The C-terminus may interact with HDAC-dependent and HDAC-independent corepressors.
  • 翻译后修饰
    Acetylated.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 130 kDa Sin3-associated polypeptide antibody
    • FLJ12761 antibody
    • Histone deacetylase complex subunit SAP130 antibody
    • KIAA0017 antibody
    • mSin3A associated protein 130 antibody
    • Pre mRNA splicing factor SF3b 130 kDa subunit antibody
    • RSE1 antibody
    • SAP 130 antibody
    • Sap130 antibody
    • SF3b 130 antibody
    • SF3b130 antibody
    • SF3B3 antibody
    • Sin3-associated polypeptide p130 antibody
    • Sin3A associated protein 130kDa antibody
    • Sin3A associated protein antibody
    • SP130_HUMAN antibody
    • Spliceosome associated protein 130 antibody
    • Splicing factor 3b subunit 3 130kDa antibody
    • Splicing factor 3b subunit 3 antibody
    • STAF 130 antibody
    • STAF130 antibody
    see all

图片

  • Anti-SAP130 antibody (ab111739) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size : 110 kDa
  • Immunofluorescence analysis of SAP130 in paraformaldehyde-fixed MCF7, using ab111739 at a 1/500 dilution. Lower image was costained with Hoechst 33342.
  • Immunohistochemical analysis of SAP130 in paraffin embedded PC14 xenograft, using ab111739 at a 1/500 dilution.

文献

ab111739 has not yet been referenced specifically in any publications.

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