概述

  • 产品名称Anti-SAP1抗体
  • 描述
    兔多克隆抗体to SAP1
  • 特异性This antibody detects SAP1 GST-fusion protein (~60 kDa band on Western Blot). Some cross reactivity is observed with GLEPP1 (PTP RO) and LAR (PTP RF).
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide of Human SAP1.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab12556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500. Detects SAP1 fusion protein as expected at ~60 kDa (predicted molecular weight of native protein: 122 kDa).

    Can be blocked with SAP1 peptide.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能May contribute to contact inhibition of cell growth and motility by mediating the dephosphorylation of focal adhesion-associated substrates and thus negatively regulating integrin-promoted signaling processes. Induces apoptotic cell death by at least two distinct mechanisms: inhibition of cell survival signaling mediated by PI 3-kinase, Akt, and ILK and activation of a caspase-dependent proapoptotic pathway. Inhibits the basal activity of LCK and its activation in response to TCR stimulation and TCR-induced activation of MAP kinase and surface expression of CD69. Inhibits TCR-induced tyrosine phosphorylation of LAT and ZAP70. Inhibits both basal activity of DOK1 and its CD2-induced tyrosine phosphorylation. Induces dephosphorylation of p130cas, focal adhesion kinase and c-Src. Reduces migratory activity of Jurkat cells.
    • 组织特异性Expressed at high levels in the brain, spleen and liver and at lower levels in the heart and stomach. Expressed in pancreatic and colorectal cancer cells, but not in normal pancreas or colon. Expression in hepatocellular carcinoma is related to the differentiation status of the tumor and expression is inversely related to tumor aggressiveness.
    • 序列相似性Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.
      Contains 8 fibronectin type-III domains.
      Contains 1 tyrosine-protein phosphatase domain.
    • 结构域The extracellular domain mediates homodimerization. One or more cysteines in the extracellular domain is essential for the formation of dimers probably by forming a disulfide bond.
      The cytoplasmic domain mediates the interaction with LCK.
    • 细胞定位Membrane. Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BEM 2 antibody
      • BEM2 antibody
      • Brain-enriched membrane-associated protein tyrosine phosphatase 2 antibody
      • Phosphotyrosine phosphatase antibody
      • Protein tyrosine phosphatase receptor type H antibody
      • PTPASE antibody
      • PTPRH antibody
      • PTPRH_HUMAN antibody
      • R-PTP-H antibody
      • Receptor-type tyrosine-protein phosphatase H antibody
      • SAP-1 antibody
      • SAP1 antibody
      • Stomach cancer-associated protein tyrosine phosphatase 1 antibody
      • Transmembrane-type protein tyrosine phosphatase H antibody
      • Transmembrane-type protein-tyrosine phosphatase type H antibody
      see all

    Anti-SAP1 antibody 图像

    • All lanes : Anti-SAP1 antibody (ab12556) at 1/500 dilution

      Lane 1 : SAP 1 (PTP RH)
      Lane 2 : PTPIA2 beta (PTP RN2)
      Lane 3 : GLEPP1 (PTP RO)
      Lane 4 : LAR (PTP RF)

      Lysates/proteins at 2.8 µg per lane.


      Predicted band size : 122 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds

      The ~60 kDa band corresponds to the size of an SAP1 GST-fusion protein. Reactivity is also observed with GLEPP1 (PTP RO) and LAR (PTP RF). 122kDa is the predicted molecular weight of native SAP1.

      Phosphatase domain fusion proteins provided by Purely Proteins

    • Anti-SAP1 antibody (ab12556) at 1/500 dilution + Whole cel lysates prepared from human Huh-7 cells at 20 µg

      Secondary
      HRP-conjugated sheep polyclonal to rabbit IgG at 1/20000 dilution

      Performed under reducing conditions.

      Predicted band size : 122 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 200 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 4 minutes

      This image is courtesy of an anonymous Abreview.

      See Abreview

    Anti-SAP1 antibody (ab12556)参考文献

    ab12556 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (huh-7)
    Loading amount 20 µg
    Specification huh-7
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 22 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Lung and breast cell lines)
    Loading amount 15 µg
    Specification Lung and breast cell lines
    Blocking step Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.05 % Twe
    Username

    Abcam user community

    Verified customer

    提交于 May 25 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"