Anti-SAMD3抗体(ab122028)
Key features and details
- Rabbit polyclonal to SAMD3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-SAMD3抗体 -
描述
兔多克隆抗体to SAMD3 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to amino acids 221-303 of Human SAMD3 isoform 1 (Q8N6K7)
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阳性对照
- Human pancreas tissue. Total protein lysate from RT-4 and U-251 MG cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122028于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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说明 |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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序列相似性
Contains 1 SAM (sterile alpha motif) domain. - Information by UniProt
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数据库链接
- Entrez Gene: 154075 Human
- Entrez Gene: 268288 Mouse
- Entrez Gene: 685940 Rat
- SwissProt: Q8N6K7 Human
- SwissProt: Q8C4H2 Mouse
- Unigene: 440508 Human
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别名
- SAM domain-containing protein 3 antibody
- SAMD3 antibody
- SAMD3_HUMAN antibody
see all
图片
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Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) -
ab122028 at 1/40 dilution staining SAMD3 in paraffin embedded Human pancreas tissue. This image shows strong cytoplasmic positivity in exocrine glandular cells.
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All lanes : Anti-SAMD3 antibody (ab122028) at 1/250 dilution
Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2 : RT-4
Lane 3 : U-251 MG
Lane 4 : Human Plasma
Lane 5 : Human Liver
Lane 6 : Human Tonsil
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab122028 被引用在 5 文献中.
- Cheng Y et al. MicroRNA-30e regulates TGF-ß-mediated NADPH oxidase 4-dependent oxidative stress by Snai1 in atherosclerosis. Int J Mol Med 43:1806-1816 (2019). PubMed: 30816428
- Luo K et al. MiR-149-5p regulates cisplatin chemosensitivity, cell growth, and metastasis of oral squamous cell carcinoma cells by targeting TGFß2. Int J Clin Exp Pathol 12:3728-3739 (2019). PubMed: 31933761
- Zhang J et al. Knockdown of vascular cell adhesion molecule 1 impedes transforming growth factor beta 1-mediated proliferation, migration, and invasion of endometriotic cyst stromal cells. Reprod Biol Endocrinol 17:69 (2019). PubMed: 31443713
- Lu W et al. AMD3100 Attenuates Post-Traumatic Osteoarthritis by Maintaining Transforming Growth Factor-ß1-Induced Expression of Tissue Inhibitor of Metalloproteinase-3 via the Phosphatidylinositol 3-Kinase/Akt Pathway. Front Pharmacol 10:1554 (2019). PubMed: 32038242
- Liu M et al. Overexpression of miR-422a inhibits cell proliferation and invasion, and enhances chemosensitivity in osteosarcoma cells. Oncol Rep 36:3371-3378 (2016). PubMed: 27779704