antigen sequence: MFTELRSKLS PPRGRAGAVR AGFGERRDVD ATAHFSFCRT LLEHTVSAES IPCHLPRTPG TS, corresponding to N terminal amino acids 1-62 of Human SAMD10.
- Human urinary bladder tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Contains 1 SAM (sterile alpha motif) domain.
Information by UniProt
- C20orf136 antibody
- SAM domain-containing protein 10 antibody
- SAM10_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-SAMD10 antibody (ab122682)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122682 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD10 antibody (ab122682)
ab122682, at 1/20 dilution, staining SAMD10 in paraffin-embedded Human urinary bladder tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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