概述

  • 产品名称Anti-SAM68抗体[EPR3231]
    参阅全部 SAM68 一抗
  • 描述
    兔单克隆抗体[EPR3231] to SAM68
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues on the N terminal of human SAM68

  • 阳性对照
    • HeLa and Jurkat cell lysates and human brain tissue and Hela cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab76471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/20000 - 1/100000. Detects a band of approximately 68 kDa (predicted molecular weight: 49 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export.
      Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase.
    • 组织特异性Ubiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver.
    • 序列相似性Belongs to the KHDRBS family.
      Contains 1 KH domain.
    • 发展阶段Isoform 3 is only expressed in growth-arrested cells.
    • 结构域The KH domain is required for binding to RNA.
      The Pro-rich domains are flanked by Arg/Gly-rich motifs which can be asymmetric dimethylated on arginine residues to give the DMA/Gly-rich regions. Selective methylation on these motifs can modulate protein-protein interactions.
    • 翻译后修饰Tyrosine phosphorylated by several non-receptor tyrosine kinases, for example LCK, FYN and JAK3. Negatively correlates with ability to bind RNA but required for many interactions with proteins.
      Acetylated. Positively correlates with ability to bind RNA.
      Arginine methylation is required for nuclear localization. Also can affect interaction with other proteins. Inhibits interaction with Src-like SH3 domains, but not interaction with WW domains of WBP4/FBP21 AND FNBP4/FBP30.
      Arg-291, Arg-331 and Arg-346 are found to be also dimethylated, probably to asymmetric dimethylarginine.
    • 细胞定位Nucleus. Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ34027 antibody
      • GAP associated tyrosine phosphoprotein p62 antibody
      • GAP-associated tyrosine phosphoprotein p62 antibody
      • KH domain containing RNA binding signal transduction associated 1 antibody
      • KH domain-containing antibody
      • KHDR1_HUMAN antibody
      • Khdrbs1 antibody
      • p21 Ras GTPase activating protein associated p62 antibody
      • p21 Ras GTPase-activating protein-associated p62 antibody
      • p62 antibody
      • p68 antibody
      • RNA-binding antibody
      • Sam68 antibody
      • signal transduction-associated protein 1 antibody
      • Src associated in mitosis 68 kDa protein antibody
      • Src-associated in mitosis 68 kDa protein antibody
      see all

    Anti-SAM68 antibody [EPR3231] 图像

    • All lanes : Anti-SAM68 antibody [EPR3231] (ab76471) at 1/100000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 49 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    • ab76471 at 1/100 dilution staining SAM68 in human brain tissue, using a HRP/AP polymerized secondary antibody.
    • Immunofluorescent staining of HeLa cells using ab76471 at a 1/100 dilution.

    Anti-SAM68 antibody [EPR3231] (ab76471)参考文献

    This product has been referenced in:
    • Rajanala K & Nandicoori VK Localization of nucleoporin Tpr to the nuclear pore complex is essential for Tpr mediated regulation of the export of unspliced RNA. PLoS One 7:e29921 (2012). WB ; Human . Read more (PubMed: 22253824) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Human Fibroblast)
    Loading amount 20 µg
    Specification Human Fibroblast
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Nupage gel)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    提交于 Jan 09 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"