Anti-SAM68抗体(ab86239)

概述

  • 产品名称Anti-SAM68抗体
    参阅全部 SAM68 一抗
  • 描述
    兔多克隆抗体to SAM68
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1 and 50 of Human SAM68 (NP_006550.1).

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab86239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export.
    Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase.
  • 组织特异性Ubiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver.
  • 序列相似性Belongs to the KHDRBS family.
    Contains 1 KH domain.
  • 发展阶段Isoform 3 is only expressed in growth-arrested cells.
  • 结构域The KH domain is required for binding to RNA.
    The Pro-rich domains are flanked by Arg/Gly-rich motifs which can be asymmetric dimethylated on arginine residues to give the DMA/Gly-rich regions. Selective methylation on these motifs can modulate protein-protein interactions.
  • 翻译后修饰Tyrosine phosphorylated by several non-receptor tyrosine kinases, for example LCK, FYN and JAK3. Negatively correlates with ability to bind RNA but required for many interactions with proteins.
    Acetylated. Positively correlates with ability to bind RNA.
    Arginine methylation is required for nuclear localization. Also can affect interaction with other proteins. Inhibits interaction with Src-like SH3 domains, but not interaction with WW domains of WBP4/FBP21 AND FNBP4/FBP30.
    Arg-291, Arg-331 and Arg-346 are found to be also dimethylated, probably to asymmetric dimethylarginine.
  • 细胞定位Nucleus. Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ34027 antibody
    • GAP associated tyrosine phosphoprotein p62 antibody
    • GAP-associated tyrosine phosphoprotein p62 antibody
    • KH domain containing RNA binding signal transduction associated 1 antibody
    • KH domain-containing antibody
    • KHDR1_HUMAN antibody
    • Khdrbs1 antibody
    • p21 Ras GTPase activating protein associated p62 antibody
    • p21 Ras GTPase-activating protein-associated p62 antibody
    • p62 antibody
    • p68 antibody
    • RNA-binding antibody
    • Sam68 antibody
    • signal transduction-associated protein 1 antibody
    • Src associated in mitosis 68 kDa protein antibody
    • Src-associated in mitosis 68 kDa protein antibody
    see all

Anti-SAM68 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SAM68 with ab86239 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-SAM68 antibody (ab86239) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 49 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 250 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 seconds
  • Detection of SAM68 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86239 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 1 second.

Anti-SAM68 antibody (ab86239)参考文献

ab86239 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application CLIP
Sample Human Cell lysate - whole cell (HEK 293T)
Specification HEK 293T
Type iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control hnRNP C (sc-15386) bound protein A dynabeads 0.1 mg/ml (antibody/beads)
Negative control Protein A dynabeads without antibody bound
Immuno-precipitation step Other - Protein A dynabeads
Username

Abcam user community

Verified customer

提交于 Jun 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"