Anti-Salmonella serogroup E抗体[42-77] (ab8144)

概述

  • 产品名称Anti-Salmonella serogroup E抗体[42-77]
  • 描述
    小鼠单克隆抗体[42-77] to Salmonella serogroup E
  • 特异性The antibody reacts with 9 out of 9 tested Salmonella (belonging to serogroup E): The monoclonal reacts with: S. Meleagridis S. Anatum S. Newbrunswick S. Goerlitz S. Canoga S. Minneapolis S. Taksony S. Senftenberg S. Niloese It shows only minor reactivity with a few members of 140 tested non-Salmonella bacteria. No reactivity with other Salmonella serogroups from group A, B, C1, C2, D, K and Y was observed (40 tested).
  • 经测试应用适用于: Agglutination, ELISAmore details
  • 常规说明


    The antibody reacts with bacteria in suspension and is therefore useful for detection of Salmonella in bacterial suspensions. Various systems can be used with this antibody to detect the presence of Samonella.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 纯度Tissue culture supernatant
  • 纯化说明Ammonium sulphate precipitation followed by Mono Q-column.
  • Primary antibody说明The antibody reacts with bacteria in suspension and is therefore useful for detection of Salmonella in bacterial suspensions. Various systems can be used with this antibody to detect the presence of Samonella.
  • 克隆单克隆
  • 克隆编号42-77
  • 同种型IgG3
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Agglutination Use at an assay dependent concentration.

Prepare bacteria suspension in a physiological salt solution and place 10 µl on glass slide. Add 10 µl antibody solution, mix well and watch clumping. Incorporate always negative and positive controls.

ELISA 1/10.

Dilute in PBS containing 4% BSA + 0.05% Tween. Prepare bacteria suspension in PBS (5x108 bact/ml). Coat ELISA plate with 100 ul/well for 1 hour at 37°C, store overnight at 4°C. Wash 5x with tapwater with 0.05% Tween (TT). Incubate with diluted antibody, 1 hour at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density.

Anti-Salmonella serogroup E antibody [42-77] (ab8144)参考文献

ab8144 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I apologise for the slight delay in response as in this instance it has been necessary to contact the originator of the clone itself to gain this information. The monoclonal reacts with: S. Meleagridis S. Anatum S. New...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"