Anti-SALL1抗体[K9814] (ab41974)

概述

  • 产品名称
    Anti-SALL1抗体[K9814]
    参阅全部 SALL1 一抗
  • 描述
    小鼠单克隆抗体[K9814] to SALL1
  • 特异性
    This antibody is specific for SALL1.
  • 经测试应用
    适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Baculovirus-expressed recombinant fragment, corresponding to amino acids 258/499 of Human SALL1

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab31526 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab41974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA Use a concentration of 0.5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 140 kDa.

靶标

  • 功能
    Transcriptional repressor involved in organogenesis.
  • 组织特异性
    Highest levels in kidney. Lower levels in adult brain (enriched in corpus callosum, lower expression in substantia nigra) and liver.
  • 疾病相关
    Defects in SALL1 are the cause of Townes-Brocks syndrome (TBS) [MIM:107480]. TBS is a rare, autosomal dominant malformation syndrome with a combination of imperforate anus, triphalangeal and supernumerary thumbs, malformed ears and sensorineural hearing loss.
    Defects in SALL1 may cause a phenotype overlapping with TBS, similar to bronchio-oto-renal syndrome (BOR) [MIM:113650]. BOR is an autosomal dominant disorder, manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to Mondini type cochlear defect and stapes fixation.
  • 序列相似性
    Belongs to the sal C2H2-type zinc-finger protein family.
    Contains 9 C2H2-type zinc fingers.
  • 发展阶段
    In fetal brain exclusively in neurons of the subependymal region of hypothalamus lateral to the third ventricle.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HSal1 antibody
    • Sal like protein 1 antibody
    • Sal-1 antibody
    • Sal-like protein 1 antibody
    • Sal1 antibody
    • SALL1 antibody
    • SALL1_HUMAN antibody
    • Spalt-like transcription factor 1 antibody
    • TBS antibody
    • Zinc finger protein 794 antibody
    • Zinc finger protein SALL1 antibody
    • Zinc finger protein Spalt-1 antibody
    see all

图片

  • Ab41974 staining SALL1 in Mouse embryonic kidney at tissue sections by Immunohistochemistry (IHC- P- paraformaldehyde-fixed, paraffin- embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10µg/ml BSA for 1 hour at 25oC; antigen retrieval was by heat mediation in a Tris/EDTA (pH9).  Samples were incubated with primary antibody (1/100 with 6% Horse serum in PBST) for 16hours at 4oC. An Alexa Fluor® 568 donkey anti-mouse polyclonal (1/500 dilution) was used as the secondary antibody.

    See Abreview

文献

ab41974 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse embryonic kidney at 13.5 dpc)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA ph9
Permeabilization
No
Specification
Mouse embryonic kidney at 13.5 dpc
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
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提交于 Sep 16 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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