概述

  • 产品名称
  • 描述
    兔多克隆抗体to SALL1
  • 经测试应用
    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Chicken, Human, Drosophila melanogaster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Human SALL1.

    (Peptide available as ab31903.)

  • 阳性对照
    • The antibody gave a positive signal in E14Tg2a cell lysate. The antibody gave a positive signal in the following methanol fixed cell line: Mouse embryonic stem cells.

性能

应用

Our Abpromise guarantee covers the use of ab31526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 140 kDa).

靶标

  • 功能
    Transcriptional repressor involved in organogenesis.
  • 组织特异性
    Highest levels in kidney. Lower levels in adult brain (enriched in corpus callosum, lower expression in substantia nigra) and liver.
  • 疾病相关
    Defects in SALL1 are the cause of Townes-Brocks syndrome (TBS) [MIM:107480]. TBS is a rare, autosomal dominant malformation syndrome with a combination of imperforate anus, triphalangeal and supernumerary thumbs, malformed ears and sensorineural hearing loss.
    Defects in SALL1 may cause a phenotype overlapping with TBS, similar to bronchio-oto-renal syndrome (BOR) [MIM:113650]. BOR is an autosomal dominant disorder, manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to Mondini type cochlear defect and stapes fixation.
  • 序列相似性
    Belongs to the sal C2H2-type zinc-finger protein family.
    Contains 9 C2H2-type zinc fingers.
  • 发展阶段
    In fetal brain exclusively in neurons of the subependymal region of hypothalamus lateral to the third ventricle.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HSal1 antibody
    • Sal like protein 1 antibody
    • Sal-1 antibody
    • Sal-like protein 1 antibody
    • Sal1 antibody
    • SALL1 antibody
    • SALL1_HUMAN antibody
    • Spalt-like transcription factor 1 antibody
    • TBS antibody
    • Zinc finger protein 794 antibody
    • Zinc finger protein SALL1 antibody
    • Zinc finger protein Spalt-1 antibody
    see all

图片

  • Anti-SALL1 antibody (ab31526) at 1 µg/ml + E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 140 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 130 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    Although the predicted band size is 140 kDa based on Swiss-prot data, a band of 200 kDa has been previously observed. J Biol Chem. 2011 Jan 14;286(2):1037-45 PMID:21062744

  • ab31526 staining SALL1 in metanephric blastema cells, murine kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ICC/IF image of ab31526 stained Mouse embryonic stem cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31526 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Reginensi A  et al. A critical role for NF2 and the Hippo pathway in branching morphogenesis. Nat Commun 7:12309 (2016). IF . Read more (PubMed: 27480037) »
  • Kumar N  et al. Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells. Elife 4:N/A (2015). Read more (PubMed: 26554899) »

See all 6 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Frontal cortex)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Frontal cortex
Blocking step
FBS as blocking agent for 5 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 17 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Tissue (imaginal disc)
Specification
imaginal disc
Username

Abcam user community

Verified customer

提交于 Sep 03 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Abcam user community

Verified customer

提交于 Dec 17 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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