antigen sequence, corresponding to internal sequence amino acids 486-630 of Human SAGE1.
- Human testis tissue; Human U 2 OS cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
Expressed mainly in bladder, lung, head and neck carcinomas. Not expressed in normal tissues except for testis.
Information by UniProt
- Cancer/testis antigen 14 antibody
- CT14 antibody
- RP11-97N5.1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAGE1 antibody (ab121175)
ab121175 at 1/250 dilution staining SAGE1 in Paraffin-embedded Human Testis tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-SAGE1 antibody (ab121175)
ab121175 at 4 µg/ml staining SAGE1 in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).
has not yet been referenced specifically in any publications.
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