antigen sequence, corresponding to amino acids 247-387 of Human SAGE1.
- Human spleen tissue; Human U 2 OS cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/350. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
Expressed mainly in bladder, lung, head and neck carcinomas. Not expressed in normal tissues except for testis.
Information by UniProt
- Cancer/testis antigen 14 antibody
- CT14 antibody
- RP11-97N5.1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAGE1 antibody (ab121160)
ab121160 at 1/350 dilution staining SAGE1 in Paraffin-embedded Human Spleen tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-SAGE1 antibody (ab121160)
ab121160 at 4 µg/ml staining SAGE1 in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SAGE1 antibody(ab121160)
Immunohistochemical staining of Human testis shows strong nuclear positivity in cells in seminiferus ducts using ab121160
has not yet been referenced specifically in any publications.
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