Mouse, Rat, Cow
Recombinant protein fragment corresponding to a region within amino acids 476-705 of Human SAFB (NP_002958).
A431, H1299, HeLa and HepG2 cell lysates for WB; HeLa cells for ICC/IF
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 103 kDa.
1/100 - 1/200.
Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation.
Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SAP domain.
Information by UniProt
glutathione S transferase fusion protein antibody
Western blot - SAFB antibody (ab97520)
Anti-SAFB antibody (ab97520) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 103 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - SAFB antibody (ab97520)
ab97520, at a 1/100 dilution, staining SAFB in paraformaldehyde fixed HeLa cells by Immunofluorescence analysis.
Lower image was merged with DNA probe.
has not yet been referenced specifically in any publications.
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