Anti-SAFB抗体[4i59] (ab18086)

概述

  • 产品名称Anti-SAFB抗体[4i59]
    参阅全部 SAFB 一抗
  • 描述
    小鼠单克隆抗体[4i59] to SAFB
  • 特异性Ab18086 recognizes HET/SAFB, Mr 130kDa; and a protein, Mr 34kDa.
  • 经测试应用适用于: WB, IP, ICC, IHC-Pmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Synthetic peptide:

    CPEARDKEDGRKF

    , corresponding to amino acids 345 to 357 of SAFB.

  • 表位The immunizing sequence has 8/11 identical amino acids in mouse and human transcription factor tbx2 (T-box protein 2).
  • 阳性对照
    • An independent laboratory has shown HET/SAFB to be immunoprecipitated from MCF-7 and HepG2 cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号4i59
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab18086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 102 kDa.
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.

靶标

  • 功能Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation.
  • 组织特异性Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.
  • 序列相似性Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SAP domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp779C1727 antibody
    • glutathione S transferase fusion protein antibody
    • HAP antibody
    • HET antibody
    • HSP27 ERE TATA binding protein antibody
    • HSP27 ERE-TATA-binding protein antibody
    • HSP27 estrogen response element-TATA box-binding protein antibody
    • SAF B antibody
    • SAF-B antibody
    • SAF-B1 antibody
    • SAFB 1 antibody
    • SAFB antibody
    • SAFB1 antibody
    • SAFB1_HUMAN antibody
    • Scaffold attachment factor B1 antibody
    see all

Anti-SAFB antibody [4i59] (ab18086)参考文献

ab18086 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I can tell you that the tissue tested in IHC was the human cell line MCF-7 human breast adenocarcinoma cell line and also the human hepatocellular liver carcinoma cell line HepG2. I have no information available on an ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"