Anti-SAE2 / UBA2抗体(ab95835)
Key features and details
- Rabbit polyclonal to SAE2 / UBA2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-SAE2 / UBA2抗体
参阅全部 SAE2 / UBA2 一抗 -
描述
兔多克隆抗体to SAE2 / UBA2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken, Cow, Dog, Zebrafish -
免疫原
Synthetic peptide corresponding to Human SAE2/ UBA2 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab109093) -
阳性对照
- This antibody gave a positive signal in both Human brain and Human heart tissue lysates aswell as the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293; Caco2.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95835于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
|
说明 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). |
靶标
-
功能
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. -
通路
Protein modification; protein sumoylation. -
序列相似性
Belongs to the ubiquitin-activating E1 family. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 10054 Human
- Entrez Gene: 50995 Mouse
- Entrez Gene: 308508 Rat
- Entrez Gene: 406672 Zebrafish
- Omim: 613295 Human
- SwissProt: Q9UBT2 Human
- SwissProt: Q9Z1F9 Mouse
- SwissProt: Q7SXG4 Zebrafish
see all -
别名
- Anthracycline associated resistance ARX antibody
- Anthracycline-associated resistance ARX antibody
- ARX antibody
see all
图片
-
All lanes : Anti-SAE2 / UBA2 antibody (ab95835) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 6 : Human brain tissue lysate - total protein (ab29466)
Lane 7 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 20 minutes
Abcam recommends using milk as the blocking agent at a concentration of 3%. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab95835 尚未被引用在任何文献中。