Anti-SAE2 / UBA2抗体(ab58451)

概述

  • 产品名称Anti-SAE2 / UBA2抗体
    参阅全部 SAE2 / UBA2 一抗
  • 描述
    兔多克隆抗体to SAE2 / UBA2
  • 特异性Detects endogenous levels of total Uba2 protein.
  • 经测试应用适用于: ELISA, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from C-terminal of human Uba2.

  • 阳性对照
    • Extracts from 293 cells, human lung carcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab58451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
  • 通路Protein modification; protein sumoylation.
  • 序列相似性Belongs to the ubiquitin-activating E1 family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Anthracycline associated resistance ARX antibody
    • Anthracycline-associated resistance ARX antibody
    • ARX antibody
    • FLJ13058 antibody
    • HRIHFB2115 antibody
    • SAE 2 antibody
    • SAE2 antibody
    • SAE2_HUMAN antibody
    • SUMO 1 activating enzyme subunit 2 antibody
    • SUMO activating enzyme subunit 2 antibody
    • SUMO-activating enzyme subunit 2 antibody
    • UBA2 antibody
    • UBA2 ubiquitin activating enzyme E1 homolog antibody
    • Ubiquitin like 1 activating enzyme E1B antibody
    • Ubiquitin like modifier activating enzyme 2 antibody
    • Ubiquitin-like 1-activating enzyme E1B antibody
    • UBLE1B antibody
    see all

Anti-SAE2 / UBA2 antibody 图像

  • All lanes : Anti-SAE2 / UBA2 antibody (ab58451) at 1/500 dilution

    Lane 1 : extracts from 293 cells treated with UV (5mins)
    Lane 2 : extracts from 293 cells treated with UV (5mins) with immunizing peptide


    Predicted band size : 71 kDa
    Observed band size : 71 kDa
  • Ab58451 at 1/50-1/100 dilution staining human lung carcinoma without and with blocking peptide; paraffin embedded.
  • ICC/IF image of ab58451 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58451, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SAE2 / UBA2 antibody (ab58451)参考文献

ab58451 has not yet been referenced specifically in any publications.

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