Mouse, Rat, Cow
Recombinant fragment, corresponding to a region within amino acids 3-217 of Human SAE1 (AAH00344)
293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cells, A549 Xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 38 kDa.
1/100 - 1/250.
1/100 - 1/200.
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Expression level increases during S phase and drops in G2 phase (at protein level).
Protein modification; protein sumoylation.
Belongs to the ubiquitin-activating E1 family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Activator of SUMO1 antibody
Western blot - Anti-SAE1 antibody (ab97523)
All lanes : Anti-SAE1 antibody (ab97523) at 1/1000 dilution Lane 1 : A431 whole cell lysate Lane 2 : H1299 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 38 kDa 10% SDS-PAGE.
Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody (ab97523)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97523 at 1/200 dilution. Lower image is merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE1 antibody (ab97523)
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using ab97523 at 1/100 dilution.
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