概述

  • 产品名称Anti-SAE1抗体
    参阅全部 SAE1 一抗
  • 描述
    兔多克隆抗体to SAE1
  • 经测试应用适用于: IP, IHC-Pmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 296-346 of Human SAE1 (NP_005491.1).

  • 阳性对照
    • HeLa whole cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab103522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
    • 组织特异性Expression level increases during S phase and drops in G2 phase (at protein level).
    • 通路Protein modification; protein sumoylation.
    • 序列相似性Belongs to the ubiquitin-activating E1 family.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Activator of SUMO1 antibody
      • AOS1 antibody
      • HSPC140 antibody
      • Sae1 antibody
      • SAE1_HUMAN antibody
      • Sentrin/SUMO activating protein AOS1 antibody
      • SUA1 antibody
      • SUMO 1 activating enzyme E1 N subunit antibody
      • SUMO 1 activating enzyme subunit 1 antibody
      • SUMO-activating enzyme subunit 1 antibody
      • Ubiquitin like protein SUMO1 activating enzyme antibody
      • Ubiquitin-like 1-activating enzyme E1A antibody
      • UBL E1A antibody
      • UBLE1A antibody
      see all

    Anti-SAE1 antibody 图像

    • Detection of SAE1 by Western Blot of Immunprecipitate.
      ab103522 at 0.4µg/ml staining SAE1 in HeLa whole cell lysate immunoprecipitated using ab103522 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SAE1 with ab103522 at 1/1000 (1µg/ml). Detection: DAB.

    Anti-SAE1 antibody (ab103522)参考文献

    ab103522 has not yet been referenced specifically in any publications.

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