概述

  • 产品名称Anti-Sacsin抗体
    参阅全部 Sacsin 一抗
  • 描述
    兔多克隆抗体to Sacsin
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Sacsin.

  • 阳性对照
    • This antibody gave a positive signal in human hippocampus formalin fixed paraffin embedded tissue section.

性能

应用

Our Abpromise guarantee covers the use of ab80195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Co-chaperone which acts as a regulator of the Hsp70 chaperone machinery and may be involved in the processing of other ataxia-linked proteins.
  • 组织特异性Highly expressed in the central nervous system. Also found in skeletal muscle and at low levels in pancreas.
  • 疾病相关Spastic ataxia Charlevoix-Saguenay type
  • 序列相似性Contains 1 HEPN domain.
    Contains 1 J domain.
    Contains 1 ubiquitin-like domain.
  • 结构域The ubiquitin-like domain mediates interaction with the proteasome.
    The J domain is functional and is shown to stimulate E.coli dnaK ATPase activity.
  • 细胞定位Cytoplasm. Predominantly cytoplasmic, a small portion is present in the nucleus and also shows a partial mitochondrial overlap with the mitochondrial marker Hsp60.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DnaJ homolog subfamily C member 29 antibody
    • DNAJC29 antibody
    • SACS antibody
    • SACS_HUMAN antibody
    • Sacsin antibody
    • Spastic ataxia of Charlevoix-Saguenay antibody
    see all

Anti-Sacsin antibody 图像

  • IHC image of Sacsin staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80195, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • IF image of ab80195 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80195, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

Anti-Sacsin antibody (ab80195)参考文献

ab80195 has not yet been referenced specifically in any publications.

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