antigen sequence, corresponding to amino acids 268-376 of Human SAC3D1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Involved in centrosome duplication and mitotic progression.
Cytoplasm; cytoskeleton; centrosome By similarity. Cytoplasm; cytoskeleton; spindle By similarity. Note: Localizes on centrosomes in interphase cells and at spindles in mitosis
- HSU79266 antibody
- SAC3 domain-containing protein 1 antibody
- SAC3 homology domain-containing protein 1 antibody
- SHD1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-SAC3D1 antibody (ab122809)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122809 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAC3D1 antibody (ab122809)
ab122809, at 1/200 dilution, staining SAC3D1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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